Back to Search
Start Over
Autophagy Alteration in ApoA-I Related Systemic Amyloidosis
- Source :
- International Journal of Molecular Sciences; Volume 23; Issue 7; Pages: 3498, E-Prints Complutense. Archivo Institucional de la UCM, instname
- Publication Year :
- 2022
-
Abstract
- Amyloidoses are characterized by the accumulation and aggregation of misfolded proteins into fibrils in different organs, leading to cell death and consequent organ dysfunction. The specific substitution of Leu 75 for Pro in Apolipoprotein A-I protein sequence (ApoA-I; L75P-ApoA-I) results in late onset amyloidosis, where deposition of extracellular protein aggregates damages the normal functions of the liver. In this work, we describe that the autophagic process is inhibited in the presence of the L75P-ApoA-I amyloidogenic variant in stably transfected human hepatocyte carcinoma cells. The L75P-ApoA-I amyloidogenic variant alters the redox status of the cells, resulting into excessive mitochondrial stress and consequent cell death. Moreover, L75P-ApoA-I induces an impairment of the autophagic flux. Pharmacological induction of autophagy or transfection-enforced overexpression of the pro-autophagic transcription factor EB (TFEB) restores proficient proteostasis and reduces oxidative stress in these experimental settings, suggesting that pharmacological stimulation of autophagy could be a promising target to alleviate ApoA-I amyloidosis.
- Subjects :
- Gastroenterología y hepatología
Apolipoprotein A-I
Organic Chemistry
Apoptosi
General Medicine
Amyloidosis
Catalysis
Computer Science Applications
Inorganic Chemistry
Protein Aggregates
amyloidosis
autophagy
apoptosis
apolipoprotein A-I
Amyloidosi
Autophagy
Humans
lipids (amino acids, peptides, and proteins)
Immunoglobulin Light-chain Amyloidosis
Physical and Theoretical Chemistry
Molecular Biology
Spectroscopy
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 23
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- International journal of molecular sciences
- Accession number :
- edsair.doi.dedup.....307a7651d040903108c47a5893732689