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13,13a-seco-protoberberines from the tubers of Corydalis yanhusuo and their anti-inflammatory activity
- Source :
- Phytochemistry. 194
- Publication Year :
- 2021
-
Abstract
- Six undescribed protoberberine derivatives including two pairs of enantiomers, named yanhusanines G-L, along with fifteen reported protoberberine alkaloids, were isolated from the tubers of Corydalis yanhusuo. Among them, yanhusanines H-L feature a unique 13,13a-seco skeleton which is rare in nature. Their structural elucidations were achieved by extensive spectroscopic analysis and quantum chemistry calculations. A biogenetic route for yanhusanines H-L was proposed. Bioassay results showed that yanhusanine J exhibited potent inhibitory effect against the nitric oxide (NO) production in lipopolysaccharide (LPS) induced RAW 264.7 cells (IC50 = 2.25 ± 1.32 μM). Western blot analysis demonstrated that yanhusanine J exerted its anti-inflammatory effect via suppressing the nuclear factor kappa B (NF-κB) pathway, together with the decrease of the inflammatory factors TNF-α, IL-6 and IL-1β. Furthermore, molecular simulation docking indicated that yanhusanine J had strong interaction with the active site of the inducible nitric oxide synthase (iNOS) protein.
- Subjects :
- medicine.drug_class
Stereochemistry
ved/biology.organism_classification_rank.species
Berberine Alkaloids
Anti-Inflammatory Agents
Plant Science
Horticulture
Biochemistry
Anti-inflammatory
Nitric oxide
chemistry.chemical_compound
Western blot
medicine
Papaveraceae
Molecular Biology
Taxonomy
medicine.diagnostic_test
biology
ved/biology
Active site
Biodiversity
General Medicine
biology.organism_classification
Nitric oxide synthase
chemistry
Corydalis
Docking (molecular)
biology.protein
Corydalis yanhusuo
Subjects
Details
- ISSN :
- 18733700
- Volume :
- 194
- Database :
- OpenAIRE
- Journal :
- Phytochemistry
- Accession number :
- edsair.doi.dedup.....30a276ec559676704e19471d6f26d00c