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Broccoli Florets Supplementation Improves Insulin Sensitivity and Alters Gut Microbiome Population—A Steatosis Mice Model Induced by High-Fat Diet
- Source :
- Frontiers in Nutrition, Frontiers in Nutrition, Vol 8 (2021)
- Publication Year :
- 2021
- Publisher :
- Frontiers Media SA, 2021.
-
Abstract
- Nonalcoholic fatty liver disease (NAFLD) is linked to obesity, type 2 diabetes, hyperlipidemia, and gut dysbiosis. Gut microbiota profoundly affects the host energy homeostasis, which, in turn, is affected by a high-fat diet (HFD) through the liver-gut axis, among others. Broccoli contains beneficial bioactive compounds and may protect against several diseases. This study aimed to determine the effects of broccoli supplementation to an HFD on metabolic parameters and gut microbiome in mice. Male (7–8 weeks old) C57BL/J6 mice were divided into four groups: normal diet (ND), high-fat diet (HFD), high-fat diet+10% broccoli florets (HFD + F), and high-fat diet + 10% broccoli stalks (HFD + S). Liver histology and serum biochemical factors were evaluated. Alterations in protein and gene expression of the key players in lipid and carbohydrate metabolism as well as in gut microbiota alterations were also investigated. Broccoli florets addition to the HFD significantly reduced serum insulin levels, HOMA-IR index, and upregulated adiponectin receptor expression. Conversely, no significant difference was found in the group supplemented with broccoli stalks. Both broccoli stalks and florets did not affect fat accumulation, carbohydrate, or lipid metabolism-related parameters. Modifications in diversity and in microbial structure of proteobacteria strains, Akermansia muciniphila and Mucispirillum schaedleri were observed in the broccoli-supplemented HFD-fed mice. The present study suggests that dietary broccoli alters parameters related to insulin sensitivity and modulates the intestinal environment. More studies are needed to confirm the results of this study and to investigate the mechanisms underlying these beneficial effects.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Normal diet
Endocrinology, Diabetes and Metabolism
Population
gut microbiome
Gut flora
Carbohydrate metabolism
03 medical and health sciences
0302 clinical medicine
NAFLD
Internal medicine
lipid metabolism
Nonalcoholic fatty liver disease
medicine
insulin sensitivity
TX341-641
education
Nutrition
Original Research
broccoli
Adiponectin receptor 1
education.field_of_study
Nutrition and Dietetics
biology
Nutrition. Foods and food supply
digestive, oral, and skin physiology
nutritional and metabolic diseases
food and beverages
Lipid metabolism
medicine.disease
biology.organism_classification
high-fat diet
030104 developmental biology
Endocrinology
030211 gastroenterology & hepatology
Steatosis
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 2296861X
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Frontiers in Nutrition
- Accession number :
- edsair.doi.dedup.....30c73b5fc645e105eed57866d812c957
- Full Text :
- https://doi.org/10.3389/fnut.2021.680241