Additional file 2 of 2-Pentadecyl-2-oxazoline ameliorates memory impairment and depression-like behaviour in neuropathic mice: possible role of adrenergic alpha2- and H3 histamine autoreceptors

Additional file 2: Figure S2: Effect of PEA-OXA in COS cells stably expressing human histamine H4 and H1 receptors. (A) Scatter plots showing the effect of PEA-OXA in COS cells expressing histamine H4 receptors on intracellular cAMP levels. (B) Bar graph showing the quantification of [Ca2 +]i measurements performed in histamine H1 receptor transfected COS cells. (C, D) Representative images showing the Ca2+ oscillations in COS H1 cells following the preincubation with PEA-OXA 5 µM and stimulation with histamine 100 µM and/or thapsigargin 5 µM. (D) Data represent the mean ± SEM of ≥ 5 determinations. Data sets were compared by use of one-way ANOVA followed by Bonferroni’s test. The asterisk denotes a p value ≤ 0.05.