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The Location of Plastocyanin in Vascular Plant Photosystem I
- Source :
- Journal of Biological Chemistry. 277:25692-25696
- Publication Year :
- 2002
- Publisher :
- Elsevier BV, 2002.
-
Abstract
- We have studied the binding sites of the electron donor and acceptor proteins of vascular plant photosystem I by electron microscopy/crystallography. Previously, we identified the binding site for the electron acceptor (ferredoxin). In this paper we complete these studies with the characterization of the electron donor (plastocyanin) binding site. After cross-linking, plastocyanin is detected using Fourier difference analysis of two dimensionally ordered arrays of photosystem I located at the periphery of chloroplast grana. Plastocyanin binds in a small cavity on the lumenal surface of photosystem I, close to the center and with a slight bias toward the PsaL subunit of the complex. The recent release of the full coordinates for the cyanobacterial photosystem I reaction center has allowed a detailed comparison between the structures of the eukaryotic and prokaryotic systems. This reveals a very close homology, which is particularly striking for the lumenal side of photosystem I.
- Subjects :
- chemistry.chemical_classification
Photosynthetic reaction centre
Binding Sites
P700
Photosystem I Protein Complex
Cytochrome b6f complex
Photosynthetic Reaction Center Complex Proteins
food and beverages
macromolecular substances
Cell Biology
Electron acceptor
Biology
Photosystem I
Biochemistry
Chloroplast
Microscopy, Electron
Crystallography
chemistry
Spinacia oleracea
Ferredoxins
Plastocyanin
Molecular Biology
Ferredoxin
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 277
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....30f319ce4e4205016bcad7f9c9d4b8c9