Localized electroporation with track-etched membranes

In PNAS, Cao et al. (1) employ track-etched membranes for the intracellular delivery of various molecular cargoes via electroporation in both adherent and suspended cells. While the concept of utilizing such membranes for electroporation is not novel (2, 3), its extension to suspended cells and the wide parametric space covered in the article are noteworthy. The objectives of this letter are to correct a minor lapse in the article’s introduction, as well as to put the results in a broader context. In ref. 1, the authors cite that the delivery efficiency of a plasmid, using track-etched membranes and localized electroporation (2), was less than 50% (reference 16 in ref. 1). However, an efficiency … [↵][1]1To whom correspondence may be addressed. Email: espinosa{at}northwestern.edu. [1]: #xref-corresp-1-1