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Investigation by Synchrotron Radiation Circular Dichroism of the Secondary Structure Evolution of Pepsin under Oxidative Environment
- Source :
- Foods, Foods, MDPI, 2021, 10 (5), pp.998. ⟨10.3390/foods10050998⟩, Foods, 2021, 10 (5), pp.998. ⟨10.3390/foods10050998⟩, Foods, Vol 10, Iss 998, p 998 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- International audience; Food processing affects the structure and chemical state of proteins. In particular, protein oxidation occurs and may impair protein properties. These chemical reactions initiated during processing can develop during digestion. Indeed, the physicochemical conditions of the stomach (oxygen pressure, low pH) favor oxidation. In that respect, digestive proteases may be affected as well. Yet, very little is known about the link between endogenous oxidation of digestive enzymes, their potential denaturation, and, therefore, food protein digestibility. Thus, the objective of this study is to understand how oxidative chemical processes will impact the pepsin secondary structure and its hydrolytic activity. The folding and unfolding kinetics of pepsin under oxidative conditions was determined using Synchrotron Radiation Circular Dichroism. SRCD gave us the possibility to monitor the rapid kinetics of protein folding and unfolding in real-time, giving highly resolved spectral data. The proteolytic activity of control and oxidized pepsin was investigated by MALDI-TOF mass spectrometry on a meat protein model, the creatine kinase. MALDI-TOF MS allowed a rapid evaluation of the proteolytic activity through peptide fingerprint. This study opens up new perspectives by shifting the digestion paradigm taking into account the gastric digestive enzyme and its substrate.
- Subjects :
- 0301 basic medicine
Health (social science)
Plant Science
TP1-1185
digestion
Protein oxidation
01 natural sciences
Health Professions (miscellaneous)
Microbiology
03 medical and health sciences
Pepsin
Denaturation (biochemistry)
protein oxidation
Protein secondary structure
synchrotron radiation circular dichroism
pepsin
mass spectrometry
2. Zero hunger
chemistry.chemical_classification
biology
010405 organic chemistry
Chemical technology
Communication
0104 chemical sciences
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition
030104 developmental biology
Enzyme
chemistry
Digestive enzyme
Biophysics
biology.protein
Protein folding
Digestion
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 23048158
- Volume :
- 10
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Foods
- Accession number :
- edsair.doi.dedup.....312e660b7c3b58320ed7058317abbc5d