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Influence of biological stimuli on the phase behaviour of a biomedical thermoresponsive polymer: A comparative investigation of hemeproteins
- Source :
- Journal of colloid and interface science. 541
- Publication Year :
- 2018
-
Abstract
- Hypothesis Thorough knowledge of the biomolecular interactions between thermoresponsive polymers (TRPs) and proteins are necessary to fabricate potential materials for various bio-related science and technology applications. Although over the past four decades have evidenced that tremendous growth in the utilization of TRPs in the addition of various stimuli systems, a underlying molecular origins of the role of biological stimuli on TRPs phase transition is still missing. Experiments We demonstrated the comparative effect of heme proteins (biological stimuli) such as cytochrome c (Cyt c) and myoglobin (Mb) on the phase behaviour of poly(N-isopropylacrylamide) (PNIPAM) aqueous solution. The sophisticated multiple techniques have confirmed that the structural integrity of the proteins is found to play a vital role in altering the phase transition temperature of PNIPAM to different extents with increasing the concentration of proteins. Findings With the addition of Cyt c, the lower critical solution temperature (LCST) of PNIPAM (33.0 °C) increased towards higher temperature (35.7 °C) in contrast to this with the addition of Mb the LCST of PNIPAM decreased towards lower temperature (30.7 °C). This discrepancy is due to the difference in contrast structural arrangements of both proteins on the LCST of PNIPAM. On the basis of these results, the variation in the structures of both heme proteins, hydration and dehydration between the heme proteins are compared. These results demonstrated that depending on the type of smart polymers used for bio-related applications, it is necessary to take into account the effect of biological stimuli while designing polymers. We hope that the present study can stimulate more novel concepts and dramatic changes in smart polymer and protein interactions.
- Subjects :
- Hemeproteins
Hemeprotein
Polymers
02 engineering and technology
010402 general chemistry
01 natural sciences
Lower critical solution temperature
Smart polymer
Phase Transition
Protein–protein interaction
Biomaterials
Colloid and Surface Chemistry
Phase (matter)
Humans
Thermoresponsive polymers in chromatography
chemistry.chemical_classification
biology
Cytochrome c
Temperature
Water
Polymer
021001 nanoscience & nanotechnology
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
chemistry
Biophysics
biology.protein
0210 nano-technology
Subjects
Details
- ISSN :
- 10957103
- Volume :
- 541
- Database :
- OpenAIRE
- Journal :
- Journal of colloid and interface science
- Accession number :
- edsair.doi.dedup.....3139b3a4cf092fedb4f97ef6224def35