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An enzyme-free surface plasmon resonance imaging biosensing method for highly sensitive detection of microRNA based on catalytic hairpin assembly and spherical nucleic acid
- Source :
- Analytica chimica acta. 1108
- Publication Year :
- 2019
-
Abstract
- MicroRNAs (miRNAs), considered as therapeutic targets and biomarkers, play important roles in biological processes. Herein, an enzyme-free surface plasmon resonance imaging (SPRi) biosensing method has been developed for miRNA detection based on catalytic hairpin assembly and spherical nucleic acid. The hairpin H1 tethered on the surface of the sensor chip is unfolded by miRNA, and then the hybridized miRNA is released through the displacement of the hairpin H2 for the successive hybridization and assembly process. The emerging DNA fragments on the sensor chip surface after hairpins assembly are further used to hybridize with spherical nucleic acid, inducing a remarkably amplified SPR signal. This biosensing method is highly sensitive to miRNA with a detection limit of 53.7 fM and a linear range of 4 orders of magnitude. Moreover, the biosensor demonstrates good specificity and has the ability to distinguish members of homologous miRNA family even with single base differences. Thus, the SPRi biosensing method may hold a great promise for further application in early clinical diagnosis.
- Subjects :
- 02 engineering and technology
Biosensing Techniques
01 natural sciences
Biochemistry
Analytical Chemistry
Catalysis
chemistry.chemical_compound
Limit of Detection
microRNA
Surface plasmon resonance imaging
Environmental Chemistry
Animals
Spectroscopy
010401 analytical chemistry
Inverted Repeat Sequences
Nucleic Acid Hybridization
Reproducibility of Results
DNA
Surface Plasmon Resonance
021001 nanoscience & nanotechnology
0104 chemical sciences
Highly sensitive
MicroRNAs
chemistry
Linear range
Spherical nucleic acid
Biophysics
Cattle
0210 nano-technology
Biosensor
Subjects
Details
- ISSN :
- 18734324
- Volume :
- 1108
- Database :
- OpenAIRE
- Journal :
- Analytica chimica acta
- Accession number :
- edsair.doi.dedup.....313ad5cd1050891f1b56482dff0ed7a8