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Digitonin-sensitive LHCII enlarges the antenna of Photosystem I in stroma lamellae of Arabidopsis thaliana after far-red and blue-light treatment
- Source :
- Biochimica et Biophysica Acta-Bioenergetics 1860 (2019) 8, Biochimica et Biophysica Acta-Bioenergetics, 1860(8), 651-658
- Publication Year :
- 2019
-
Abstract
- Light drives photosynthesis. In plants it is absorbed by light-harvesting antenna complexes associated with Photosystem I (PSI) and photosystem II (PSII). As PSI and PSII work in series, it is important that the excitation pressure on the two photosystems is balanced. When plants are exposed to illumination that overexcites PSII, a special pool of the major light-harvesting complex LHCII is phosphorylated and moves from PSII to PSI (state 2). If instead PSI is over-excited the LHCII complex is dephosphorylated and moves back to PSII (state 1). Recent findings have suggested that LHCII might also transfer energy to PSI in state 1. In this work we used a combination of biochemistry and (time-resolved) fluorescence spectroscopy to investigate the PSI antenna size in state 1 and state 2 for Arabidopsis thaliana. Our data shows that 0.7 ± 0.1 unphosphorylated LHCII trimers per PSI are present in the stroma lamellae of state-1 plants. Upon transition to state 2 the antenna size of PSI in the stroma membrane increases with phosphorylated LHCIIs to a total of 1.2 ± 0.1 LHCII trimers per PSI. Both phosphorylated and unphosphorylated LHCII function as highly efficient PSI antenna.
- Subjects :
- 0106 biological sciences
0301 basic medicine
ITIS Middelware
Photosystem II
Light
Arabidopsis
Light-Harvesting Protein Complexes
Biophysics
Digitonin
Photosynthesis
Photosystem I
01 natural sciences
Biochemistry
Fluorescence spectroscopy
Light-harvesting complex
03 medical and health sciences
Arabidopsis thaliana
Phosphorylation
Photosystem
VLAG
biology
Photosystem I Protein Complex
Chemistry
IT Design
Excitation-energy transfer
Photosystem II Protein Complex
Far-red
Time-resolved fluorescence
Cell Biology
biology.organism_classification
State transition
030104 developmental biology
Spectrometry, Fluorescence
Biofysica
Energy Transfer
EPS
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 00052728
- Volume :
- 1860
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta - Bioenergetics
- Accession number :
- edsair.doi.dedup.....315785081449b38bd8e559dfc373aaa3
- Full Text :
- https://doi.org/10.1016/j.bbabio.2019.07.001