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Recruitment of HU by piggyback: a special role of GalR in repressosome assembly

Authors :
Sudeshna Kar
Sankar Adhya
Publication Year :
2001
Publisher :
Cold Spring Harbor Laboratory Press, 2001.

Abstract

In Gal repressosome assembly, a DNA loop is formed by the interaction of two GalR, bound to two distal operators, and the binding of the histone-like protein, HU, to an architecturally critical position on DNA to facilitate the GalR–GalR interaction. We show that GalR piggybacks HU to the critical position on the DNA through a specific GalR–HU interaction. This is the first example of HU making a specific contact with another protein. The GalR–HU contact that results in cooperative binding of the two proteins to DNA may be transient and absent in the final repressosome structure. A sequence-independent DNA-binding protein being recruited to an architectural site on DNA through a specific association with a regulatory protein may be a common mode for assembly of complex nucleoprotein structures.

Details

Language :
English
Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....31621328c9448c2ede08c05e0aea5763