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Recruitment of HU by piggyback: a special role of GalR in repressosome assembly
- Publication Year :
- 2001
- Publisher :
- Cold Spring Harbor Laboratory Press, 2001.
-
Abstract
- In Gal repressosome assembly, a DNA loop is formed by the interaction of two GalR, bound to two distal operators, and the binding of the histone-like protein, HU, to an architecturally critical position on DNA to facilitate the GalR–GalR interaction. We show that GalR piggybacks HU to the critical position on the DNA through a specific GalR–HU interaction. This is the first example of HU making a specific contact with another protein. The GalR–HU contact that results in cooperative binding of the two proteins to DNA may be transient and absent in the final repressosome structure. A sequence-independent DNA-binding protein being recruited to an architectural site on DNA through a specific association with a regulatory protein may be a common mode for assembly of complex nucleoprotein structures.
- Subjects :
- Models, Molecular
Transcription, Genetic
Protein Conformation
Blotting, Western
Repressor
Plasma protein binding
Biology
DNA-binding protein
Models, Biological
Chromosomes
chemistry.chemical_compound
Protein structure
Transcription (biology)
Genetics
Escherichia coli
A-DNA
Amino Acids
Promoter Regions, Genetic
Glucuronidase
Cell Nucleus
Dose-Response Relationship, Drug
Models, Genetic
Escherichia coli Proteins
Cooperative binding
DNA
Precipitin Tests
Cell biology
DNA-Binding Proteins
Repressor Proteins
chemistry
Mutation
Mutagenesis, Site-Directed
Dimerization
Developmental Biology
Research Paper
Plasmids
Protein Binding
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....31621328c9448c2ede08c05e0aea5763