Back to Search
Start Over
Co-expression of Dorsal and Rel2 Negatively Regulates Antimicrobial Peptide Expression in the Tobacco Hornworm Manduca sexta
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Nature Publishing Group, 2016.
-
Abstract
- Nuclear factor κB (NF-κB) plays an essential role in regulation of innate immunity. In mammals, NF-κB factors can form homodimers and heterodimers to activate gene expression. In insects, three NF-κB factors, Dorsal, Dif and Relish, have been identified to activate antimicrobial peptide (AMP) gene expression. However, it is not clear whether Dorsal (or Dif) and Relish can form heterodimers. Here we report the identification and functional analysis of a Dorsal homologue (MsDorsal) and two Relish short isoforms (MsRel2A and MsRel2B) from the tobacco hornworm, Manduca sexta. Both MsRel2A and MsRel2B contain only a Rel homology domain (RHD) and lack the ankyrin-repeat inhibitory domain. Overexpression of the RHD domains of MsDorsal and MsRel2 in Drosophila melanogaster S2 and Spodoptera frugiperda Sf9 cells can activate AMP gene promoters from M. sexta and D. melanogaster. We for the first time confirmed the interaction between MsDorsal-RHD and MsRel2-RHD, and suggesting that Dorsal and Rel2 may form heterodimers. More importantly, co-expression of MsDorsal-RHD with MsRel2-RHD suppressed activation of several M. sexta AMP gene promoters. Our results suggest that the short MsRel2 isoforms may form heterodimers with MsDorsal as a novel mechanism to prevent over-activation of antimicrobial peptides.
- Subjects :
- 0301 basic medicine
Gene isoform
medicine.medical_specialty
Antimicrobial peptides
Spodoptera
Article
Rel homology domain
03 medical and health sciences
0302 clinical medicine
Protein Domains
Internal medicine
Manduca
Gene expression
medicine
Animals
Promoter Regions, Genetic
Regulation of gene expression
Multidisciplinary
biology
fungi
NF-kappa B
biology.organism_classification
3. Good health
Cell biology
030104 developmental biology
Endocrinology
Drosophila melanogaster
Gene Expression Regulation
Manduca sexta
Insect Proteins
030217 neurology & neurosurgery
Antimicrobial Cationic Peptides
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....316db6e75c8ae7cc52ddbac606a59384