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Hyaluronan stimulates ex vivo B lymphocyte chemotaxis and cytokine production in a murine model of fungal allergic asthma
- Source :
- Immunobiology. 220:899-909
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Allergic asthma is a chronic inflammatory disease of the airways characterized by excessive eosinophilic and lymphocytic inflammation with associated changes in the extracellular matrix (ECM) resulting in airway wall remodeling. Hyaluronan (HA) is a nonsulfated glycosaminoglycan ECM component that functions as a structural cushion in its high molecular mass (HMM) but has been implicated in metastasis and other disease processes when it is degraded to smaller fragments. However, relatively little is known about the role HA in mediating inflammatory responses in allergy and asthma. In the present study, we used a murine Aspergillus fumigatus inhalational model to mimic human disease. After observing in vivo that a robust B cell recruitment followed a massive eosinophilic egress to the lumen of the allergic lung and corresponded with the detection of low molecular mass HA (LMM HA), we examined the effect of HA on B cell chemotaxis and cytokine production in the ex vivo studies. We found that LMM HA functioned through a CD44-mediated mechanism to elicit chemotaxis of B lymphocytes, while high molecular mass HA (HMM HA) had little effect. LMM HA, but not HMM HA, also elicited the production of IL-10 and TGF-β1 in these cells. Taken together, these findings demonstrate a critical role for ECM components in mediating leukocyte migration and function which are critical to the maintenance of allergic inflammatory responses.
- Subjects :
- Male
Leukocyte migration
Antigens, Fungal
B cell chemotaxis
medicine.medical_treatment
Immunology
Biology
Article
Transforming Growth Factor beta1
Mice
chemistry.chemical_compound
Lymphocyte chemotaxis
Hyaluronic acid
medicine
Animals
Immunology and Allergy
Lymphocyte Count
Hyaluronic Acid
B-Lymphocytes
Aspergillus fumigatus
Chemotaxis
Hematology
Immunoglobulin E
Asthma
Extracellular Matrix
Interleukin-10
Mice, Inbred C57BL
Disease Models, Animal
Interleukin 10
Hyaluronan Receptors
Cytokine
chemistry
Female
Bronchoalveolar Lavage Fluid
Ex vivo
Subjects
Details
- ISSN :
- 01712985
- Volume :
- 220
- Database :
- OpenAIRE
- Journal :
- Immunobiology
- Accession number :
- edsair.doi.dedup.....319a1bb7ccab3e3d2c7cb18ab8ac7853
- Full Text :
- https://doi.org/10.1016/j.imbio.2015.01.011