Figure S5. ChIP-seq analysis of FLAG-H1.3 in OV-3/H1.3(H) cells. from Histone H1.3 Suppresses H19 Noncoding RNA Expression and Cell Growth of Ovarian Cancer Cells

Figure S5. ChIP-seq analysis of FLAG-H1.3 in OV-3/H1.3(H) cells. A) Metagene analysis of H1.3 distribution on genes partitioned by gene expression levels (each group containing 20% of genes in genome) over a 10 kb region covering -5 kb to +5 kb of transcription start sites (TSS). On average, H1.3 exhibits the deepest dip at TSS of highly expressed genes (red) but no or minimal dip at TSS of inactive genes (blue). ChIP-seq was performed using anti-FLAG antibody in OV-3/H1.3(H) cells. B) Distribution of H1.3 along H19 (upper) and GAPDH (lower) loci in OV-3/H1.3(H) cells. Y axis: tag counts per 1000 bp window per 10 million mappable reads. IP-IN: normalized signal intensity of ChIP-seq subtracted by that of input-seq.