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Comparison of liver and plasma metabolic profiles in piglets of different ages as animal models for paediatric population
- Source :
- The Analyst
- Publication Year :
- 2020
- Publisher :
- Royal Society of Chemistry (RSC), 2020.
-
Abstract
- Liver plays an important role in drug metabolism, so studying the grade of maturation of this organ would help to develop more appropriate dosing regimens for paediatric populations. Nevertheless, considering the invasive nature of liver analyses there are obvious ethical boundaries, particularly in babies and children. In this work, we investigated the suitability of blood plasma as an alternative matrix to evaluate the biological age of liver. With this aim, we studied the correlation of plasma and liver metabolomic profiles obtained by HPLC-TOF-MS for piglets of different ages (newborns, neonates and infants). By means of Pearson correlation analysis we observed that 360 and 1784 pairs of metabolite features were significantly correlated in positive and negative ionization mode, respectively. Procrustes analysis was applied in order to assess the similarity of the clustering resulting from the data obtained from the two matrices and the two ionisation modes. The Procrustes distances were low for both ESI+ (0.3753) and ESI- (0.3673) and, hence, liver and plasma are expected to provide similar discriminatory information. Furthermore, we found that Multiblock Principal Component Analysis (MB-PCA) readily allowed us to combine the data obtained from both matrices and to better understand the clustering according to the three study groups. Considering all these results, we suggest that plasma can provide valuable insight into the maturation grade of liver in order to provide accurate dosing in paediatric population.
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
Swine
Metabolite
Physiology
Biology
01 natural sciences
Biochemistry
Analytical Chemistry
Correlation
Plasma
03 medical and health sciences
chemistry.chemical_compound
Metabolomics
Blood plasma
Electrochemistry
Metabolome
Animals
Humans
Environmental Chemistry
Child
Spectroscopy
030304 developmental biology
0303 health sciences
010401 analytical chemistry
Infant, Newborn
Infant
3. Good health
0104 chemical sciences
Liver
Pharmaceutical Preparations
chemistry
Models, Animal
Principal component analysis
Procrustes analysis
Drug metabolism
Subjects
Details
- ISSN :
- 13645528 and 00032654
- Volume :
- 145
- Database :
- OpenAIRE
- Journal :
- The Analyst
- Accession number :
- edsair.doi.dedup.....31c319e8e39a088ffbf41e21e59015a2