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Effects of insulin-like growth factor-1 on the properties of mesenchymal stem cells in vitro
- Source :
- Journal of Zhejiang University SCIENCE B. 13:20-28
- Publication Year :
- 2012
- Publisher :
- Zhejiang University Press, 2012.
-
Abstract
- Objective: To explore the effects of insulin-like growth factor-1 (IGF-1) on migration, proliferation and differentiation of mesenchymal stem cells (MSCs). Methods: MSCs were obtained from Sprague-Dawley rats by a combination of gradient centrifugation and cell culture techniques and treated with IGF-1 at concentrations of 5–20 ng/ml. Proliferation of MSCs was determined as the mean doubling time. Expression of CXC chemokine receptor 4 (CXCR4) and migration property were determined by flow cytometry and transwell migration essay, respectively. mRNA expression of GATA-4 and collagen II was determined by reverse transcription-polymerase chain reaction (RT-PCR). Results: The mean doubling time of MSC proliferation was decreased, and the expression of CXCR4 on MSCs and migration of MSCs were increased by IGF-1, all in a dose-dependent manner, while the optimal concentration of IGF-1 on proliferation and migration was different. IGF-1 did not affect the expression of GATA-4 or collagen II mRNA. Conclusions: IGF-1 dose-dependently stimulated the proliferation of MSCs, upregulated the expression of CXCR4, and accelerated migration. There was no apparent differentiation of MSCs to cardiomyocytes or chondrocytes after culturing with IGF-1 alone.
- Subjects :
- Receptors, CXCR4
medicine.medical_treatment
Cellular differentiation
Biology
General Biochemistry, Genetics and Molecular Biology
Rats, Sprague-Dawley
Insulin-like growth factor
Cell Movement
medicine
Animals
Doubling time
RNA, Messenger
CXC chemokine receptors
Insulin-Like Growth Factor I
General Pharmacology, Toxicology and Pharmaceutics
Collagen Type II
Stem cell transplantation for articular cartilage repair
Dose-Response Relationship, Drug
General Veterinary
Reverse Transcriptase Polymerase Chain Reaction
Mesenchymal stem cell
Cell Differentiation
Mesenchymal Stem Cells
General Medicine
Flow Cytometry
Molecular biology
GATA4 Transcription Factor
Rats
Endothelial stem cell
Biomedicine
Cell culture
Subjects
Details
- ISSN :
- 18621783 and 16731581
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Journal of Zhejiang University SCIENCE B
- Accession number :
- edsair.doi.dedup.....31d188a9f84ced0e1f5ebe588a6c101a