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Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health
- Source :
- Redox Biology, Redox Biology, Vol 10, Iss C, Pp 65-77 (2016)
- Publication Year :
- 2016
- Publisher :
- eScholarship, University of California, 2016.
-
Abstract
- Blood based bioenergetic profiling strategies are emerging as potential reporters of systemic mitochondrial function; however, the extent to which these measures reflect the bioenergetic capacity of other tissues is not known. The premise of this work is that highly metabolically active tissues, such as skeletal and cardiac muscle, are susceptible to differences in systemic bioenergetic capacity. Therefore, we tested whether the respiratory capacity of blood cells, monocytes and platelets, are related to contemporaneous respirometric assessments of skeletal and cardiac muscle mitochondria. 18 female vervet/African green monkeys (Chlorocebus aethiops sabaeus) of varying age and metabolic status were examined for this study. Monocyte and platelet maximal capacity correlated with maximal oxidative phosphorylation capacity of permeabilized skeletal muscle (R=0.75, 95% confidence interval [CI]: 0.38–0.97; R=0.51, 95%CI: 0.05–0.81; respectively), isolated skeletal muscle mitochondrial respiratory control ratio (RCR; R=0.70, 95%CI: 0.35–0.89; R=0.64, 95%CI: 0.23–0.98; respectively), and isolated cardiac muscle mitochondrial RCR (R=0.55, 95%CI: 0.22–0.86; R=0.58, 95%CI: 0.22–0.85; respectively). These results suggest that blood based bioenergetic profiling may be used to report on the bioenergetic capacity of muscle tissues. Blood cell respirometry represents an attractive alternative to tissue based assessments of mitochondrial function in human studies based on ease of access and the minimal participant burden required by these measures.<br />Graphical abstract fx1<br />Highlights • Respirometry of circulating cells and muscle tissues from non-human primates. • Blood cells recapitulate key aspects of skeletal muscle fiber bioenergetics. • Blood cells recapitulate skeletal and cardiac muscle mitochondrial bioenergetics.
- Subjects :
- 0301 basic medicine
XF, extracellular flux
Bioenergetics
BMI, body mass index
Clinical Biochemistry
OXPHOS, oxidative phosphorylation
Mitochondrion
Medical Biochemistry and Metabolomics
Cardiovascular
Biochemistry
Mitochondria, Heart
Monocytes
Oxidative Phosphorylation
Blood cell
CII, complex 2
Chlorocebus aethiops
lcsh:QH301-705.5
lcsh:R5-920
O2k, Oroboros Oxygraph 2K
Cardiac muscle
Heart
ELISA, enzyme-linked immunosorbent assay
MAS, mannitol and sucrose buffer
Skeletal
Pharmacology and Pharmaceutical Sciences
PmFBs, permeabilized fiber bundles
Mitochondria
EGTA, triethylene glycol diammine tetraacetic acid
HOMA-IR, homeostatic model assessment of insulin resistance
medicine.anatomical_structure
ACD, acid citrate dextrose
Biomarker (medicine)
Muscle
Female
RCR, respiratory control ratio
lcsh:Medicine (General)
MES, 2-(N-morpholino)ethanesulfonic acid
Research Paper
Blood Platelets
medicine.medical_specialty
Blood cells
Cellular respiration
1.1 Normal biological development and functioning
ETC, electron transport chain
Cell Respiration
Oxidative phosphorylation
Biology
03 medical and health sciences
FCCP, Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone
Oxygen Consumption
PBS, phosphate buffered saline
Underpinning research
MAX, maximal FCCP-linked bioenergetic capacity
Clinical Research
Internal medicine
medicine
CP, Chappell-Perry buffer
Animals
Muscle, Skeletal
Myocardium
Organic Chemistry
Skeletal muscle
Mitochondria, Muscle
030104 developmental biology
Endocrinology
lcsh:Biology (General)
Musculoskeletal
BSA, bovine serum albumin
Biochemistry and Cell Biology
PGE1, prostaglandin E1
SD, standard deviation
Energy Metabolism
Biomarkers
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Redox Biology, Redox Biology, Vol 10, Iss C, Pp 65-77 (2016)
- Accession number :
- edsair.doi.dedup.....31ddbcb3baa751a9e64bfe911413ec2b