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Gangliosides interact with synaptotagmin to form the high-affinity receptor complex for botulinum neurotoxin B
- Source :
- Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences of the United States of America, 2019, 116 (36), pp.18098-18108. ⟨10.1073/PNAS.1908051116⟩, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2019, 116 (36), pp.18098-18108. ⟨10.1073/PNAS.1908051116⟩
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- International audience; Botulinum neurotoxin type B (BoNT/B) recognizes nerve terminals by binding to 2 receptor components: a polysialoganglioside, predominantly GT1b, and synaptotagmin 1/2. It is widely thought that BoNT/B initially binds to GT1b then diffuses in the plane of the membrane to interact with synaptotagmin. We have addressed the hypothesis that a GT1b–synaptotagmin cis complex forms the BoNT/B receptor. We identified a consensus glycosphingolipid-binding motif in the extracellular juxtamembrane domain of synaptotagmins 1/2 and confirmed by Langmuir monolayer, surface plasmon resonance, and circular dichroism that GT1b interacts with synaptotagmin peptides containing this sequence, inducing α-helical structure. Molecular modeling and tryptophan fluorescence spectroscopy were consistent with the intertwining of GT1b and synaptotagmin, involving cis interactions between the oligosaccharide and ceramide moieties of GT1b and the juxtamembrane and transmembrane domains of synaptotagmin, respectively. Furthermore, a point mutation on synaptotagmin, located outside of the BoNT/B-binding segment, inhibited GT1b binding and blocked GT1b-induced potentiation of BoNT/B binding to synaptotagmin-expressing cells. Our findings are consistent with a model in which a preassembled GT1b–synaptotagmin complex constitutes the high-affinity BoNT/B receptor.
- Subjects :
- Protein Conformation, alpha-Helical
Ceramide
Circular dichroism
endocrine system
animal structures
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Synaptotagmin 1
Synaptotagmins
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Protein Domains
Synaptotagmin II
Animals
Botulinum Toxins, Type A
Receptor
[SDV.BC] Life Sciences [q-bio]/Cellular Biology
030304 developmental biology
0303 health sciences
Binding Sites
Multidisciplinary
Point mutation
technology, industry, and agriculture
botulinum neurotoxin B receptor
Long-term potentiation
gangliosides
Rats
Transmembrane domain
synaptotagmin
PNAS Plus
chemistry
nervous system
Synaptotagmin I
Biophysics
lipids (amino acids, peptides, and proteins)
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 00278424 and 10916490
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences of the United States of America, 2019, 116 (36), pp.18098-18108. ⟨10.1073/PNAS.1908051116⟩, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2019, 116 (36), pp.18098-18108. ⟨10.1073/PNAS.1908051116⟩
- Accession number :
- edsair.doi.dedup.....31ece8a080cc84fd202aa04b00b7394c