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The hepatoprotective effects of Pyrus biossieriana buhse leaf extract on tert-butyl hydroperoxide toxicity in HepG2 cell line
- Source :
- BMC Research Notes, Vol 14, Iss 1, Pp 1-8 (2021), BMC Research Notes
- Publication Year :
- 2021
- Publisher :
- BMC, 2021.
-
Abstract
- Objective In present study, the effects of the leaf extract of Pyrus biossieriana Buhse on tert-Butyl hydroperoxide (t-BHP) induced toxicity in the HepG2 cell line were investigated. Results HepG2 cells were exposed to different concentrations of both extract (1.5, 2.0, and 2.5 mg/mL) and t-BHP (100, 150, and 200 μM). The total flavonoid and phenolic contents, the cell viability, lipid peroxidation, NO generation, and the total antioxidant capacity in cell media were assessed. The amount of arbutin was estimated 12.6% of the dry weight of leaves (equivalent to 126 mg/g). Additionally, the amounts of flavonoids and phenols in extract were estimated 119 mg/g and 418 mg/g, respectively. The cells incubated with t-BHP showed a significant decrease in survival (p P. biossieriana Buhse leaf extract at concentrations of 1.5 and 2.0 mg/mL is capable of attenuating t-BHP-induced cytotoxicity in HepG2 cells.
- Subjects :
- Antioxidant
Science (General)
Cell Survival
QH301-705.5
medicine.medical_treatment
Cytotoxicity
Pyrus biossieriana Buhse
Flavonoid
General Biochemistry, Genetics and Molecular Biology
Pyrus
Lipid peroxidation
chemistry.chemical_compound
Q1-390
tert-Butyl hydroperoxide
tert-Butylhydroperoxide
medicine
Humans
Viability assay
Food science
Biology (General)
chemistry.chemical_classification
HepG2 cell line
Plant Extracts
Cell growth
Arbutin
Hep G2 Cells
General Medicine
Research Note
Oxidative Stress
chemistry
Toxicity
Medicine
Lipid Peroxidation
Subjects
Details
- Language :
- English
- ISSN :
- 17560500
- Volume :
- 14
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Research Notes
- Accession number :
- edsair.doi.dedup.....323699b545a7343895050a859f6ad03c