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In vivo brain imaging with multimodal optical coherence microscopy in a mouse model of thromboembolic photochemical stroke
- Source :
- Neurophotonics
-
Abstract
- We used a new multimodal imaging system that combines optical coherence microscopy and brightfield microscopy. Using this in vivo brain monitoring approach and cranial window implantation, we three-dimensionally visualized the vascular network during thrombosis, with high temporal (18 s) and spatial (axial, 2.5 μm; lateral, 2.2 μm) resolution. We used a modified mouse model of photochemical thromboembolic stroke in order to more accurately parallel human stroke. Specifically, we applied green laser illumination to focally occlude a branch of the middle cerebral artery. Despite the recanalization of the superficial arteries at 24 h after stroke, no blood flow was detected in the small vessels within deeper regions. Moreover, after 24 h of stroke progression, scattering signal enhancement was observed within the stroke region. We also evaluated the infarct extent and shape histologically. In summary, we present a novel approach for real-time mouse brain monitoring and ischemic variability analysis. This multimodal imaging method permits the analysis of thrombosis progression and reperfusion. Additionally and importantly, the system could be used to study the effect of poststroke drug treatments on blood flow in small arteries and capillaries of the brain.
- Subjects :
- Paper
cranial window
optical coherence microscopy
cerebral blood flow
Neuroscience (miscellaneous)
Thromboembolic stroke
Photochemistry
01 natural sciences
010309 optics
03 medical and health sciences
0302 clinical medicine
Neuroimaging
medicine.artery
0103 physical sciences
photochemical thromboembolic stroke
Medicine
Radiology, Nuclear Medicine and imaging
angiography
Stroke
Radiological and Ultrasound Technology
medicine.diagnostic_test
business.industry
Blood flow
in vivo mouse brain imaging
medicine.disease
Research Papers
Cerebral blood flow
Middle cerebral artery
Angiography
business
030217 neurology & neurosurgery
Preclinical imaging
Subjects
Details
- Language :
- English
- ISSN :
- 2329423X
- Volume :
- 7
- Issue :
- 01
- Database :
- OpenAIRE
- Journal :
- Neurophotonics
- Accession number :
- edsair.doi.dedup.....325fbc54ab5f51642d4c08d03cb3da1b
- Full Text :
- https://doi.org/10.1117/1.nph.7.1.015002