Nano-TiO2 Phototoxicity in Fresh and Seawater: Daphnia magna and Artemia sp. as Proxies

Nowadays, the industry is quite commonly using nanoparticles of titanium dioxide (nTiO2) especially in sunscreens, due to its higher reflective index in comparison to micron size TiO2. Its high demand causes its widespread environmental occurrence, thus damaging the environment. The aquatic ecosystems are the most vulnerable to contamination by nTiO2. Like other engineered nanoparticles, nTiO2 has demonstrated generation of reactive oxygen species (ROS) and reactive halogen species (RHS) in the aquatic environment under UV radiation. This study investigated the toxicity of nTiO2 towards two aquatic indicator organisms, one from freshwater (Daphnia magna) and the other from seawater (Artemia sp.), under simulated solar radiation (SSR). Daphnia magna and Artemia sp. were co-exposed in 16 h SSR and 8 h darkness cycles to different concentrations of nTiO2. The estimated EC50 at 48 h for D. magna was 3.16 mg nTiO2/L, whereas for A. sp. no toxic effects were observed. When we exposed these two organisms simultaneously to 48 h of prolonged SSR using higher nTiO2 concentrations, EC50 values of 7.60 mg/L and 5.59 mg/L nTiO2 for D. magna and A. sp., respectively, were obtained. A complementary bioassay was carried out with A. sp., by exposing this organism to a mixture of nTiO2 and organic UV filters (benzophenone 3 (oxybenzone, BP3), octocrylene (OC), and ethyl 4-aminobenzoate (EtPABA)), and then exposed to SSR. The results suggested that nTiO2 could potentially have negative impacts on these organisms, also this work outlines the different characteristics and interactions that may contribute to the mechanisms of environmental (in salted and freshwater) phototoxicity of nTiO2 and UV radiation, besides their interaction with organic compounds.
Authors acknowledge the financial support of the Spanish Ministry of Science and Innovation, for projects ROUSSEAU (CTM2017-89767-C3-1-R), CEX2018-000794-S, and CTQ2017-86466-R., and AGAUR-Gencat project 2017SGR131. A.C. Soler acknowledges Mexican CONACyT for her doctoral grant (Ref. 409154).