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CD39+ regulatory T cells suppress generation and differentiation of Th17 cells in human malignant pleural effusion via a LAP-dependent mechanism
- Source :
- Respiratory Research, Respiratory Research, Vol 12, Iss 1, p 77 (2011)
- Publication Year :
- 2011
-
Abstract
- Background Both regulatory T cells (Tregs) and T helper IL-17-producing cells (Th17 cells) have been found to be involved in human malignancies, however, the possible implication of Tregs in regulating generation and differentiation of Th17 cells in malignant pleural effusion remains to be elucidated. Methods The numbers of both CD39+Tregs and Th17 cells in malignant pleural effusion and peripheral blood from patients with lung cancer were determined by flow cytometry. The regulation and mechanism of Tregs on generation and differentiation of Th17 cells were explored. Results Both CD39+Tregs and Th17 cells were increased in malignant pleural effusion when compared with blood, and the numbers of CD39+Tregs were correlated negatively with those of Th17 cells. It was also noted that high levels of IL-1β, IL-6, and TGF-β1 could be observed in malignant pleural effusion when compared the corresponding serum, and that pleural CD39+Tregs could express latency-associated peptide on their surface. When naïve CD4+ T cells were cocultured with CD39+Tregs, Th17 cell numbers decreased as CD39+Treg numbers increased, addition of the anti-latency-associated peptide mAb to the coculture reverted the inhibitory effect exerted by CD39+Tregs. Conclusions Therefore, the above results indicate that CD39+Tregs inhibit generation and differentiation of Th17 cells via a latency-associated peptide-dependent mechanism.
- Subjects :
- Pulmonary and Respiratory Medicine
Adult
Lung Neoplasms
Cellular differentiation
Interleukin-1beta
chemical and pharmacologic phenomena
Biology
T-Lymphocytes, Regulatory
regulatory T cells
Immunophenotyping
Transforming Growth Factor beta1
Interleukin 21
Antigens, CD
Transforming Growth Factor beta
medicine
Malignant pleural effusion
Cytotoxic T cell
Humans
malignant pleural effusion
IL-2 receptor
Protein Precursors
Th17 cells
Cells, Cultured
Aged
Cell Proliferation
lcsh:RC705-779
CD40
transfer growth factor
Interleukin-6
Research
Apyrase
hemic and immune systems
Cell Differentiation
lcsh:Diseases of the respiratory system
Transforming growth factor beta
Middle Aged
medicine.disease
Flow Cytometry
Coculture Techniques
Pleural Effusion, Malignant
Immunology
Cancer research
biology.protein
latency-associated peptide
Peptides
Signal Transduction
Subjects
Details
- ISSN :
- 1465993X
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Respiratory research
- Accession number :
- edsair.doi.dedup.....328e50e8e09c750a9b9401f5e2a048f5