Supplementary Figure 1 from EW-7197, a Novel ALK-5 Kinase Inhibitor, Potently Inhibits Breast to Lung Metastasis

PDF - 120K, (A) Blockade of Smad2 phosphorylation by various ALK5 inhibitors. 4T1 cells starved in 0.2% HI-FBS medium for 24 h, and were treated with the indicated chemicals for 2 h with or without TGF-beta1 (2 ng/ml) in 0.2% HI-FBS medium. (B, C) Blockade of Smad2 phosphorylation by EW-7197. NMUMG (B) and MDA-MB-231 (C) cells were incubated with 0.2% HI-FBS medium for 24 h, and treated with TGF-beta1 (2 ng/ml) in the presence or absence of ALK5 inhibitors in 0.2% HI-FBS medium for 2 h. Lysates from the cells were analyzed by Western blotting. EW, SB, LY, IN indicate EW-7197, SB-505124, LY-2157299, and IN-1130, respectively.