Back to Search
Start Over
Plasmid DNA vaccination using skin electroporation promotes poly-functional CD4 T-cell responses
- Source :
- Immunology and cell biology. 89(3)
- Publication Year :
- 2010
-
Abstract
- Plasmid DNA vaccination using skin electroporation (EP) is a promising method able to elicit robust humoral and CD8(+) T-cell immune responses while limiting invasiveness of delivery. However, there is still only limited data available on the induction of CD4(+) T-cell immunity using this method. Here, we compare the ability of homologous prime/boost DNA vaccinations by skin EP and intramuscular (i.m.) injection to elicit immune responses by cytokine enzyme-linked immunosorbent spot (ELISPOT) assay, as well as study the complexity of CD4(+) T-cell responses to the human immunodeficiency virus antigen Gag, using multiparamater flow cytometry. We find that DNA vaccinations by skin EP and i.m. injection are capable of eliciting both single- and poly-functional vaccine-specific CD4(+) T cells. However, although DNA delivered by skin EP was administered at a five-fold lower dose it elicited significant increases in the magnitude of multiple-cytokine producers compared with i.m. immunization suggesting that the skin EP could provide greater poly-functional T-cell help, a feature associated with successful immune defense against infectious agents.
- Subjects :
- CD4-Positive T-Lymphocytes
medicine.medical_treatment
Immunology
Genetic Vectors
Biology
Injections, Intramuscular
gag Gene Products, Human Immunodeficiency Virus
DNA vaccination
Mice
Immune system
Antigen
Immunity
medicine
Vaccines, DNA
Immunology and Allergy
Animals
Humans
Skin
Immunity, Cellular
Mice, Inbred BALB C
ELISPOT
Electroporation
Vaccination
Cell Biology
Virology
Immunity, Humoral
Mice, Inbred C57BL
Cytokine
HIV-1
Cytokines
Female
CD8
Spleen
Plasmids
Subjects
Details
- ISSN :
- 14401711
- Volume :
- 89
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Immunology and cell biology
- Accession number :
- edsair.doi.dedup.....32d38f9ff919b024a19bf884475dbf51