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Characterization of TCHQ-induced genotoxicity and mutagenesis using the pSP189 shuttle vector in mammalian cells
- Source :
- Mutation research. 729(1-2)
- Publication Year :
- 2011
-
Abstract
- Tetrachlorohydroquinone (TCHQ) is a major toxic metabolite of the widely used wood preservative, pentachlorophenol (PCP), and it has also been implicated in PCP genotoxicity. However, the underlying mechanisms of genotoxicity and mutagenesis induced by TCHQ remain unclear. In this study, we examined the genotoxicity of TCHQ by using comet assays to detect DNA breakage and formation of TCHQ-DNA adducts. Then, we further verified the levels of mutagenesis by using the pSP189 shuttle vector in A549 human lung carcinoma cells. We demonstrated that TCHQ causes significant genotoxicity by inducing DNA breakage and forming DNA adducts. Additionally, DNA sequence analysis of the TCHQ-induced mutations revealed that 85.36% were single base substitutions, 9.76% were single base insertions, and 4.88% were large fragment deletions. More than 80% of the base substitutions occurred at G:C base pairs, and the mutations were G:C to C:G, G:C to T:A or G:C to A:T transversions and transitions. The most common types of mutations in A549 cells were G:C to A:T (37.14%) and A:T to C:G transitions (14.29%) and G:C to C:G (34.29%) and G:C to T:A (11.43%) transversions. We identified hotspots at nucleotides 129, 141, and 155 in the supF gene of plasmid pSP189. These mutation hotspots accounted for 63% of all single base substitutions. We conclude that TCHQ induces sequence-specific DNA mutations at high frequencies. Therefore, the safety of using this product would be carefully examined.
- Subjects :
- Base pair
Health, Toxicology and Mutagenesis
Genetic Vectors
Molecular Sequence Data
Biology
medicine.disease_cause
Transfection
chemistry.chemical_compound
DNA Adducts
Plasmid
Shuttle vector
Cell Line, Tumor
Chlorocebus aethiops
Genetics
medicine
Animals
Humans
Point Mutation
Molecular Biology
Gene
Vero Cells
Sequence Deletion
Mutation
Base Sequence
Mutagenesis
Sequence Analysis, DNA
Molecular biology
Hydroquinones
chemistry
Comet Assay
Genotoxicity
DNA
DNA Damage
Plasmids
Subjects
Details
- ISSN :
- 00275107
- Volume :
- 729
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Mutation research
- Accession number :
- edsair.doi.dedup.....32dfd17bbf67f6837b9367e35d7796b4