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Detrimental effects of antibiotics on mouse embryos in chromatin integrity, apoptosis and expression of zygotically activated genes
- Source :
- Zygote. 19:137-145
- Publication Year :
- 2010
- Publisher :
- Cambridge University Press (CUP), 2010.
-
Abstract
- SummaryThe effects of specific components in culture medium on embryo physiology have been extensively investigated to optimizein vitroculture systems; however, little attention has been paid to antibiotics, the reagents used most commonly in culture systems to prevent contamination. To investigate the potential effects of routine use of antibiotics on cultured embryos, mouse zygotes were cultured with or without antibiotics. In both groups, the developmental rate and cell number of blastocysts appear to be normal. The proportion of embryos with blastomere fragmentation increased slightly when embryos were cultured with antibiotics. In contrast, the presence of antibiotics in the embryo culture system significantly disturbs expression of zygotically activated genes, damages chromatin integrity and increases apoptosis of cultured embryos. These results provide evidence that, when cultured with antibiotics, embryos with normal appearance may possess intrinsic physiological and genetic abnormalities. We demonstrate that the adverse effects of antibiotics on mammalian embryos are more severe than we previously presumed and that antibiotics are not essential for sterility of embryo culture system therefore abolishing antibiotic supplementation during embryo culture.
- Subjects :
- animal structures
Zygote
medicine.drug_class
Sterility
Antibiotics
Apoptosis
Penicillins
Biology
Mice
medicine
Animals
RNA, Messenger
Fragmentation (cell biology)
Mice, Inbred ICR
Gene Expression Regulation, Developmental
Embryo
Embryo culture
Cell Biology
Blastomere
Embryo, Mammalian
Molecular biology
Chromatin
Anti-Bacterial Agents
Culture Media
Cell biology
Blastocyst
embryonic structures
Streptomycin
Developmental Biology
Subjects
Details
- ISSN :
- 14698730 and 09671994
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Zygote
- Accession number :
- edsair.doi.dedup.....33241999549f4c268e308f21a84d72d9
- Full Text :
- https://doi.org/10.1017/s0967199410000249