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A Comprehensive Analysis of FUT8 Overexpressing Prostate Cancer Cells Reveals the Role of EGFR in Castration Resistance

Authors :
Tung Shing Mamie Lih
Ruey-Bing Yang
Jianbo Pan
Mingmimg Dong
Ganglong Yang
Naseruddin Höti
Cheng Fen Tu
Michael C. Haffner
Yangying Zhou
Lijun Chen
Ashely Deng
Qing Kay Li
Hui Zhang
Source :
Cancers, Volume 12, Issue 2, Cancers, Vol 12, Iss 2, p 468 (2020)
Publication Year :
2020
Publisher :
Multidisciplinary Digital Publishing Institute, 2020.

Abstract

The emergence of castration-resistance is one of the major challenges in the management of patients with advanced prostate cancer. Although the spectrum of systemic therapies that are available for use alongside androgen deprivation for treatment of castration-resistant prostate cancer (CRPC) is expanding, none of these regimens are curative. Therefore, it is imperative to apply systems approaches to identify and understand the mechanisms that contribute to the development of CRPC. Using comprehensive proteomic approaches, we show that a glycosylation-related enzyme, alpha (1,6) fucosyltransferase (FUT8), which is upregulated in CRPC, might be responsible for resistance to androgen deprivation. Mechanistically, we demonstrated that overexpression of FUT8 resulted in upregulation of the cell surface epidermal growth factor receptor (EGFR) and corresponding downstream signaling, leading to increased cell survival in androgen-depleted conditions. We studied the coregulatory mechanisms of EGFR and FUT8 expression in CRPC xenograft models and found that castration induced FUT8 overexpression associated with increased expression of EGFR. Taken together, our findings suggest a crucial role played by FUT8 as a mediator in switching prostate cancer cells from nuclear receptor signaling (androgen receptor) to the cell surface receptor (EGFR) mechanisms in escaping castration-induced cell death. These findings have clinical implication in understanding the role of FUT8 as a master regulator of cell surface receptors in cancer-resistant phenotypes.

Details

Language :
English
ISSN :
20726694
Database :
OpenAIRE
Journal :
Cancers
Accession number :
edsair.doi.dedup.....3335b1a91f435d8c50ed9ee604052f8a
Full Text :
https://doi.org/10.3390/cancers12020468