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Multimodal, label-free fluorescence and Raman imaging of amyloid deposits in snap-frozen Alzheimer’s disease human brain tissue
- Source :
- Communications Biology, Lochocki, B, Boon, B D C, Verheul, S R, Zada, L, Hoozemans, J J M, Ariese, F & de Boer, J F 2021, ' Multimodal, label-free fluorescence and Raman imaging of amyloid deposits in snap-frozen Alzheimer’s disease human brain tissue ', Communications Biology, vol. 4, no. 1, 474 . https://doi.org/10.1038/s42003-021-01981-x, Lochocki, B, Boon, B D C, Verheul, S R, Zada, L, Hoozemans, J J M, Ariese, F & de Boer, J F 2021, ' Multimodal, label-free fluorescence and Raman imaging of amyloid deposits in snap-frozen Alzheimer’s disease human brain tissue ', Communications biology, vol. 4, no. 1, 474 . https://doi.org/10.1038/s42003-021-01981-x, Communications Biology, 4(1):474. Springer Nature, Communications biology, 4(1):474. Nature Research, Communications Biology, Vol 4, Iss 1, Pp 1-13 (2021)
- Publication Year :
- 2021
- Publisher :
- Nature Publishing Group UK, 2021.
-
Abstract
- Alzheimer’s disease (AD) neuropathology is characterized by hyperphosphorylated tau containing neurofibrillary tangles and amyloid-beta (Aβ) plaques. Normally these hallmarks are studied by (immuno-) histological techniques requiring chemical pretreatment and indirect labelling. Label-free imaging enables one to visualize normal tissue and pathology in its native form. Therefore, these techniques could contribute to a better understanding of the disease. Here, we present a comprehensive study of high-resolution fluorescence imaging (before and after staining) and spectroscopic modalities (Raman mapping under pre-resonance conditions and stimulated Raman scattering (SRS)) of amyloid deposits in snap-frozen AD human brain tissue. We performed fluorescence and spectroscopic imaging and subsequent thioflavin-S staining of the same tissue slices to provide direct confirmation of plaque location and correlation of spectroscopic biomarkers with plaque morphology; differences were observed between cored and fibrillar plaques. The SRS results showed a protein peak shift towards the β-sheet structure in cored amyloid deposits. In the Raman maps recorded with 532 nm excitation we identified the presence of carotenoids as a unique marker to differentiate between a cored amyloid plaque area versus a non-plaque area without prior knowledge of their location. The observed presence of carotenoids suggests a distinct neuroinflammatory response to misfolded protein accumulations.<br />Lochocki et al. report detection of amyloid plaques by label-free fluorescence and Raman imaging in snap-frozen frontal cortex tissue from Alzheimer’s disease cases. They identify presence of carotenoid in a cored amyloid plaque area, versus a non-plaque area, suggesting a distinct neuroinflammatory response to misfolded protein aggregations.
- Subjects :
- Male
Fluorescence-lifetime imaging microscopy
Pathology
medicine.medical_specialty
Amyloid
QH301-705.5
Biophysics
Medicine (miscellaneous)
Plaque, Amyloid
Neuropathology
Spectrum Analysis, Raman
General Biochemistry, Genetics and Molecular Biology
Article
Fluorescence imaging
Fluorescence
03 medical and health sciences
chemistry.chemical_compound
symbols.namesake
0302 clinical medicine
Alzheimer Disease
medicine
Frozen Sections
Humans
Biology (General)
030304 developmental biology
Aged
Aged, 80 and over
0303 health sciences
Chemistry
Human brain
Amyloidosis
Middle Aged
Staining
medicine.anatomical_structure
Raman spectroscopy
symbols
Thioflavin
Female
General Agricultural and Biological Sciences
030217 neurology & neurosurgery
Neuroscience
Subjects
Details
- Language :
- English
- ISSN :
- 23993642
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Communications Biology
- Accession number :
- edsair.doi.dedup.....333aaef3b1a9c89c0b7312f38860af8d