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Down‐regulated HDAC3 elevates microRNA‐495‐3p to restrain epithelial‐mesenchymal transition and oncogenicity of melanoma cells via reducing TRAF5
Down‐regulated HDAC3 elevates microRNA‐495‐3p to restrain epithelial‐mesenchymal transition and oncogenicity of melanoma cells via reducing TRAF5
- Source :
- Journal of Cellular and Molecular Medicine
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- MicroRNAs (miRNAs) are emerging biomarkers in biological processes and the role of miR‐495‐3p has been identified in melanoma, while the detailed molecular mechanisms remain to be further explored. We aim to explore the effect of histone deacetylase 3 (HDAC3) and miR‐495‐3p on epithelial‐mesenchymal transition (EMT) and oncogenicity of melanoma cells by regulating tumour necrosis factor receptor‐associated factor 5 (TRAF5). Levels of HDAC3, miR‐495‐3p and TRAF5 in melanoma tissues and pigmented nevus tissues were determined, and the predictive roles of HDAC3 and miR‐495‐3p in prognosis of melanoma patients were measured. The melanoma cells were screened and transfected with relative oligonucleotides and plasmids, and the expression of HDAC3, miR‐495‐3p and TRAF5, and phenotypes of melanoma cells were gauged by a series of assays. The relations between HDAC3 and miR‐495‐3p, and between miR‐495‐3p and TRAF5 were confirmed. HDAC3 and TRAF5 were increased while miR‐495‐3p was decreased in melanoma cells and tissues, and the low expression of miR‐495‐3p as well as high expression of HDAC3 indicated a poor prognosis of melanoma patients. Inhibited HDAC3 elevated miR‐495‐3p to suppress EMT and oncogenicity of melanoma cells by reducing TRAF5. HDAC3 particularly bound to miR‐495‐3p and TRAF5 was the target gene of miR‐495‐3p. Our results revealed that down‐regulated HDAC3 elevates miR‐495‐3p to suppress malignant phenotypes of melanoma cells by inhibiting TRAF5, thereby repressing EMT progression of melanoma cells. This study may provide novel targets for melanoma treatment.
- Subjects :
- Male
0301 basic medicine
epithelial‐mesenchymal transformation
Skin Neoplasms
Necrosis
Oncogenicity
Mice
oncogenicity
0302 clinical medicine
Cell Movement
Genes, Reporter
tumour necrosis factor receptor‐associated factor 5
Neoplasm Metastasis
Mice, Inbred BALB C
Pigmentation
Melanoma
Transfection
Prognosis
microrna‐495‐3p
Phenotype
Gene Expression Regulation, Neoplastic
Lymphatic Metastasis
030220 oncology & carcinogenesis
Molecular Medicine
Female
Original Article
medicine.symptom
Epithelial-Mesenchymal Transition
Mice, Nude
Biology
Histone Deacetylases
histone deacetylase 3
03 medical and health sciences
Cell Line, Tumor
microRNA
melanoma
medicine
Animals
Humans
Epithelial–mesenchymal transition
Cell Proliferation
TNF Receptor-Associated Factor 5
Gene Expression Profiling
Original Articles
Cell Biology
medicine.disease
HDAC3
MicroRNAs
030104 developmental biology
Cancer research
Subjects
Details
- ISSN :
- 15824934 and 15821838
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular and Molecular Medicine
- Accession number :
- edsair.doi.dedup.....336bed85c5bf2e95b803b22920a124ca
- Full Text :
- https://doi.org/10.1111/jcmm.15885