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MALS is a binding partner of IRSp53 at cell-cell contacts
- Source :
- FEBS letters. 554(1-2)
- Publication Year :
- 2003
-
Abstract
- Insulin receptor substrate p53 (IRSp53) is a key player in cytoskeletal dynamics, interacting with the actin modulators WAVE2 and Mena. Here, we identified a PDZ protein, MALS, as an IRSp53-interacting protein using a yeast two-hybrid screen. A pull-down assay showed that IRSp53 and MALS interact through the PDZ domain of MALS and the C-terminal PDZ-binding sequence of IRSp53. Their interaction in MDCK cells was also demonstrated by co-immunoprecipitation. Immunocytochemistry showed the colocalization of IRSp53 and MALS at cell–cell contacts. Cytochalasin D induced the redistribution of both proteins to the cytosol. Thus, MALS is a partner of IRSp53 anchoring the actin-based membrane cytoskeleton at cell–cell contacts.
- Subjects :
- Cytochalasin D
PDZ domain
Immunocytochemistry
Biophysics
Nerve Tissue Proteins
Cell Communication
Biology
Biochemistry
chemistry.chemical_compound
Structural Biology
Insulin receptor substrate
Two-Hybrid System Techniques
parasitic diseases
Genetics
Animals
Amino Acid Sequence
Cytoskeleton
Molecular Biology
Actin
Binding Sites
Actin modulator
Colocalization
Membrane Proteins
Cell Biology
Immunohistochemistry
Cell biology
Rats
Epithelial cell
Cytosol
Cytoskeletal Proteins
chemistry
Membrane cytoskeleton
lipids (amino acids, peptides, and proteins)
Carrier Proteins
Protein Binding
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 554
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- FEBS letters
- Accession number :
- edsair.doi.dedup.....336c654e4d73f444215de511fcfbced9