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Biocatalysts screening of Papaver bracteatum flora for thebaine transformation to codeine and morphine
- Source :
- Biocatalysis and Agricultural Biotechnology. 9:127-133
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- The aim of this study was to find biocatalyst which uses thebaine and extract of different parts of Papaver bracteatum to synthesize morphine alkaloids. The thebaine-resistant strains were obtained from microbial flora of different parts of P.bracteatum. They were purified and treated utilizing different concentrations of thebaine. Those that can grow at a concentration of over 500 µg/ml were chosen for the biotransformation experiments. Biotransformation experiments were carried out utilizing selected cells in the medium containing thebaine and/or the extract of P.bracteatum; the products of such biotransformation were extracted and the profiles of metabolites were evaluated using HPTLC, and LC/ESI-MS methods. Thereafter, the effective isolate for thebaine transformation was characterized by physiological, biochemical and biomolecular methods. The results show that among 67 isolates, 12 strains were selected using the HPTLC screening as candidates that can transform thebaine into codeine and morphine. Among them, 5 strains were identified to transform plant extract, among which, using LC/ESI-MS, a candidate was selected and identified as Bacillus sp. FAR. It can be concluded from this study, that this microbial flora candidate can transform thebaine into important narcotic drugs and it will be a valuable step in biotechnology.
- Subjects :
- 0301 basic medicine
Flora
Papaver bracteatum
Thebaine
Bioengineering
010402 general chemistry
01 natural sciences
Applied Microbiology and Biotechnology
Biotransformation Thebaine HPTLC LC/ESI-MS Papaver bracteatum
03 medical and health sciences
Biotransformation
medicine
Chromatography
biology
Chemistry
Codeine
food and beverages
biology.organism_classification
0104 chemical sciences
Transformation (genetics)
030104 developmental biology
Morphine
Narcotic drugs
Agronomy and Crop Science
Food Science
Biotechnology
medicine.drug
Subjects
Details
- ISSN :
- 18788181
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Biocatalysis and Agricultural Biotechnology
- Accession number :
- edsair.doi.dedup.....33724fa0b69ecb6b4348b2c465517fb4