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Polygala tenuifolia polysaccharide (PTP) inhibits cell proliferation by repressing Bmi-1 expression and downregulating telomerase activity
- Source :
- Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 36(4)
- Publication Year :
- 2014
-
Abstract
- In our previous study, we isolated a homogeneous polysaccharide (PTP) with antitumor activity from the roots of Polygala tenuifolia. In view of the close correlation between Bmi-1 expression and progression of ovarian cancer, we intend to elucidate the mechanism of its activity by determining the Bmi-1 expression and the telomerase activity in human ovarian carcinoma OVCAR-3 cells following treatment with PTP at three concentrations of 0.5, 1, and 2 mg/mL for 48 h. MTT and colony-forming assays revealed that PTP had a significant inhibitory effect on the cell growth and colony formation of OVCAR-3 cells. Furthermore, Western blot and real-time PCR analysis showed that PTP inhibited Bmi-1 both in protein and transcript levels. Besides, the telomerase activity in OVCAR-3 cells was also downregulated after PTP treatment for 48 h. Taken together, the inhibitory effect of PTP on the cell growth was at least in part mediated via the downregulation of Bmi-1 expression and the telomerase activity in OVCAR-3 cells, and PTP might be a new candidate for chemotherapeutic agent against human ovarian cancer.
- Subjects :
- Telomerase
animal structures
endocrine system diseases
Polygala
Polysaccharide
environment and public health
Downregulation and upregulation
Western blot
Polysaccharides
Ovarian carcinoma
Cell Line, Tumor
medicine
Humans
Cell Proliferation
chemistry.chemical_classification
Ovarian Neoplasms
Polycomb Repressive Complex 1
biology
medicine.diagnostic_test
Cell growth
Cell Cycle
General Medicine
biology.organism_classification
medicine.disease
Molecular biology
female genital diseases and pregnancy complications
Gene Expression Regulation, Neoplastic
enzymes and coenzymes (carbohydrates)
chemistry
Polygala tenuifolia
Female
Ovarian cancer
Subjects
Details
- ISSN :
- 14230380
- Volume :
- 36
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
- Accession number :
- edsair.doi.dedup.....337a6df2ca388d975c6d5d52ad2f0035