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Optical probing of sodium dynamics in neurons and astrocytes
- Source :
- NeuroImage, Neuroimage, vol. 58, no. 2, pp. 572-578
- Publication Year :
- 2011
-
Abstract
- Changes in intracellular Na(+) concentration underlie essential neurobiological processes, but few reliable tools exist for their measurement. Here we characterize a new synthetic Na(+)-sensitive fluorescent dye, Asante Natrium Green (ANG), with unique properties. This indicator was excitable in the visible spectrum and by two-photon illumination, suffered little photobleaching and located to the cytosol were it remained for long durations without noticeable unwanted effects on basic cell properties. When used in brain tissue, ANG yielded a bright fluorescent signal during physiological Na(+) responses both in neurons and astrocytes. Synchronous electrophysiological and fluorometric recordings showed that ANG produced accurate Na(+) measurement in situ. This new Na(+) indicator opens innovative ways of probing neuronal circuits.
- Subjects :
- In situ
Patch-Clamp Techniques
Cognitive Neuroscience
Sodium
chemistry.chemical_element
Action Potentials
Action Potentials/physiology
Algorithms
Animals
Astrocytes/metabolism
Cells, Cultured
Cerebral Cortex/cytology
Computer Simulation
Electrophysiological Phenomena
Fluorescent Dyes
Kinetics
Mice
Mice, Inbred C57BL
Microscopy, Confocal
Microscopy, Fluorescence
Neurons/metabolism
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Sodium/metabolism
Somatosensory Cortex/metabolism
In Vitro Techniques
03 medical and health sciences
0302 clinical medicine
Patch clamp
030304 developmental biology
Cerebral Cortex
Neurons
0303 health sciences
Chemistry
Somatosensory Cortex
Fluorescence
Photobleaching
Electrophysiology
Cytosol
Neurology
Astrocytes
Biophysics
Neuroscience
030217 neurology & neurosurgery
Intracellular
Subjects
Details
- Volume :
- 58
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- NeuroImage
- Accession number :
- edsair.doi.dedup.....337dedc2f14571037129b67d9f577273
- Full Text :
- https://doi.org/10.1016/j.neuroimage.2011.06.074