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Sperm Parameters, ASAs and Apoptosis After Processing by the Double Tube and Swim up Methods
- Source :
- American Journal of Men's Health, American Journal of Men's Health, Vol 15 (2021)
- Publication Year :
- 2021
-
Abstract
- The aim of this study was to improve the quality of semen samples by using a novel double-tube (DT) method. The DT method was developed to select sperm and compared with traditional swim-up (SU) technique for 31 semen samples. Sperm DNA integrity were tested with TUNEL and SCSA. Content of antisperm antibodies (ASA) in the semen was measured by ELISA and MAR. Levels of the caspase-3 in the sperm were assessed by western blotting. After SU and DT, 15 couples and 16 couples were underwent IVF-ET. The number of RCDs, the percentage of SDF and DFI, ASA and the level of caspase-3 were significantly decreased after DT and SU ( p = .001 and p < .001). When the DT and SU compared, there were significant changes in the number of RCD, the percentage of SDF and DFI, ASA and the level of caspase-3 ( p < 0.05-0.001). There was a higher cleavage rate ( p = .017) and a lower abortion rate ( p < .05) in DT-IVF group than in SU-IVF group. DT selection yielded spermatozoa with low RCDs, DFI, ASA, and caspase-3 which would be benefit for ART.
- Subjects :
- Adult
Male
Health (social science)
Double tube
lcsh:Medicine
Semen
Caspase 3
Apoptosis
DNA fragmentation
Andrology
03 medical and health sciences
0302 clinical medicine
Pregnancy
Male Sexual and Reproductive Health
In Situ Nick-End Labeling
Humans
reproductive and urinary physiology
swim-up
030304 developmental biology
0303 health sciences
030219 obstetrics & reproductive medicine
Chemistry
urogenital system
lcsh:R
Public Health, Environmental and Occupational Health
Middle Aged
Sperm
Spermatozoa
double tube method
Caspase-3
Female
Original Article
sperm selection
Subjects
Details
- ISSN :
- 15579891
- Volume :
- 15
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- American journal of men's health
- Accession number :
- edsair.doi.dedup.....338b83cc212d52f14b977b59810d7c42