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In Vivo Fluorescence Imaging is Well-Suited for the Monitoring of Adenovirus Directed Transgene Expression in Living Organisms

In Vivo Fluorescence Imaging is Well-Suited for the Monitoring of Adenovirus Directed Transgene Expression in Living Organisms

Authors :
Huseyin Bagci
Ahter Dilsad Sanlioglu
Thomas S. Griffith
Sevim Kahraman
Ercument Dirice
Burcak Yoldas
Metin Erkilic
Salih Sanlioglu
Source :
Molecular Imaging and Biology. 12:278-285
Publication Year :
2009
Publisher :
Springer Science and Business Media LLC, 2009.

Abstract

We tested a new light detection cooled charge-coupled device (CCD) for in vivo assessment of noninvasive, whole-body fluorescence optical imaging of adenovirus directed enhanced green fluorescent protein (AdEGFP) expression. AdEGFP was injected i.v. into BALB/c mice via tail vein. Whole-body fluorescence optical imaging of AdEGFP expression was performed using a Kodak 2000MM Image Station before and after vector administration. EGFP expression was exclusively detected around the abdominal cavity, and the fluorescent signal peaked at day 4 and then remained detectable for at least 30 days. Ex vivo fluorescence imaging confirmed that EGFP expression was restricted to the liver, and transgene expression was homogeneously diffused into all four lobes. These findings demonstrate that in vivo fluorescence imaging provides functional data indicating the approximate location, magnitude, and duration of AdEGFP expression.

Details

ISSN :
18602002 and 15361632
Volume :
12
Database :
OpenAIRE
Journal :
Molecular Imaging and Biology
Accession number :
edsair.doi.dedup.....3394228763c4e3c8a0fbd50a3bd30ddc
Full Text :
https://doi.org/10.1007/s11307-009-0260-x