Back to Search
Start Over
In Vivo Fluorescence Imaging is Well-Suited for the Monitoring of Adenovirus Directed Transgene Expression in Living Organisms
In Vivo Fluorescence Imaging is Well-Suited for the Monitoring of Adenovirus Directed Transgene Expression in Living Organisms
- Source :
- Molecular Imaging and Biology. 12:278-285
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- We tested a new light detection cooled charge-coupled device (CCD) for in vivo assessment of noninvasive, whole-body fluorescence optical imaging of adenovirus directed enhanced green fluorescent protein (AdEGFP) expression. AdEGFP was injected i.v. into BALB/c mice via tail vein. Whole-body fluorescence optical imaging of AdEGFP expression was performed using a Kodak 2000MM Image Station before and after vector administration. EGFP expression was exclusively detected around the abdominal cavity, and the fluorescent signal peaked at day 4 and then remained detectable for at least 30 days. Ex vivo fluorescence imaging confirmed that EGFP expression was restricted to the liver, and transgene expression was homogeneously diffused into all four lobes. These findings demonstrate that in vivo fluorescence imaging provides functional data indicating the approximate location, magnitude, and duration of AdEGFP expression.
- Subjects :
- Male
Cancer Research
Fluorescence-lifetime imaging microscopy
Transgene
Genetic Vectors
Green Fluorescent Proteins
Biology
Fluorescence
Adenoviridae
Cell Line
Green fluorescent protein
Viral vector
Mice
In vivo
Abdomen
Animals
Humans
Tissue Distribution
Whole Body Imaging
Radiology, Nuclear Medicine and imaging
Transgenes
Mice, Inbred BALB C
Molecular biology
Liver
Oncology
Organ Specificity
Preclinical imaging
Ex vivo
Subjects
Details
- ISSN :
- 18602002 and 15361632
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Molecular Imaging and Biology
- Accession number :
- edsair.doi.dedup.....3394228763c4e3c8a0fbd50a3bd30ddc
- Full Text :
- https://doi.org/10.1007/s11307-009-0260-x