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Expression of the TetA gene encoding TetA efflux protein in E. coli contributes to its increased bacterial resistance toward berberine
- Source :
- Journal of Asian Natural Products Research. 20:374-384
- Publication Year :
- 2017
- Publisher :
- Informa UK Limited, 2017.
-
Abstract
- Berberine (BBR) is a traditional Chinese medicine in various applications due to its antibacterial effect. Here we investigated the increased bacterial resistance of E. coli toward BBR. The median effective concentration (EC50) of BBR against E. coli was increased when TetA efflux protein (TEP) was introduced. Sixty-five percent of the intracellular BBR was expelled and molecular docking demonstrated the intensive interaction of TEP to BBR. Finally, the combined antibacterial experiment identified that BBR acted as an inhibitor of TEP in detoxification of tetracycline. TEP is the first discovered protein that was related to the bacterial susceptibility to BBR.
- Subjects :
- 0301 basic medicine
Berberine
Tetracycline
030106 microbiology
Pharmaceutical Science
Antibacterial effect
Antiporters
Analytical Chemistry
03 medical and health sciences
chemistry.chemical_compound
Antibiotic resistance
Bacterial Proteins
Drug Discovery
Escherichia coli
medicine
Gene
EC50
Pharmacology
Molecular Structure
Chemistry
Organic Chemistry
General Medicine
Molecular biology
Anti-Bacterial Agents
Molecular Docking Simulation
030104 developmental biology
Complementary and alternative medicine
Molecular Medicine
Efflux
Intracellular
medicine.drug
Subjects
Details
- ISSN :
- 14772213 and 10286020
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Journal of Asian Natural Products Research
- Accession number :
- edsair.doi.dedup.....33b9117d05fe34eb67d0f3394fddad49