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Prime-boost vaccination with recombinant protein and adenovirus-vector expressing Plasmodium vivax circumsporozoite protein (CSP) partially protects mice against Pb/Pv sporozoite challenge
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP, Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
- Publication Year :
- 2018
-
Abstract
- Vaccine development against Plasmodium vivax malaria lags behind that for Plasmodium falciparum. To narrow this gap, we administered recombinant antigens based on P. vivax circumsporozoite protein (CSP) to mice. We expressed in Pichia pastoris two chimeric proteins by merging the three central repeat regions of different CSP alleles (VK210, VK247, and P. vivax-like). The first construct (yPvCSP-AllFL) contained the fused repeat regions flanked by N- and C-terminal regions. The second construct (yPvCSP-AllCT) contained the fused repeat regions and the C-terminal domain, plus RI region. Mice were vaccinated with three doses of yPvCSP in adjuvants Poly (I:C) or Montanide ISA720. We also used replication-defective adenovirus vectors expressing CSP of human serotype 5 (AdHu5) and chimpanzee serotype 68 (AdC68) for priming mice which were subsequently boosted twice with yPvCSP proteins in Poly (I:C) adjuvant. Regardless of the regime used, immunized mice generated high IgG titres specific to all CSP alleles. After challenge with P. berghei ANKA transgenic parasites expressing Pb/PvVK210 or Pb/PvVK247 sporozoites, significant time delays for parasitemia were observed in all vaccinated mice. These vaccine formulations should be clinically tried for their potential as protective universal vaccine against P. vivax malaria.
- Subjects :
- 0301 basic medicine
Serotype
Plasmodium vivax
Genetic Vectors
Antibody Affinity
Protozoan Proteins
lcsh:Medicine
Antibodies, Protozoan
Parasitemia
medicine.disease_cause
Article
Viral vector
Adenoviridae
03 medical and health sciences
Mice
Immunogenicity, Vaccine
parasitic diseases
Malaria Vaccines
medicine
Malaria, Vivax
Animals
Amino Acid Sequence
VACINAÇÃO
lcsh:Science
Multidisciplinary
biology
Immunogenicity
lcsh:R
Plasmodium falciparum
biology.organism_classification
medicine.disease
Virology
Recombinant Proteins
3. Good health
Circumsporozoite protein
Disease Models, Animal
030104 developmental biology
Immunoglobulin G
lcsh:Q
Female
Immunization
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP, Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
- Accession number :
- edsair.doi.dedup.....33c183d1a6c830c5c1e9f429615a3729