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Determination of the antimalarial drug piperaquine in small volume pediatric plasma samples by LC–MS/MS
- Source :
- Bioanalysis, vol 6, iss 23
- Publication Year :
- 2014
- Publisher :
- Future Science Ltd, 2014.
-
Abstract
- Aim: Determination of piperaquine (PQ) in pediatric plasma requires a method with a small sample volume. Results: We report a sensitive LC–MS/MS method for quantitation of PQ with only 25 µl human plasma. Using a deuterated internal standard (PQ-d6), an analytical PFP column, APCI+ as the ion source and MRM (535/288 for PQ and 541/294 for the IS) for detection, the method has a linear calibration range of 1.5–250 ng/ml with a runtime of 3.0 min per sample. The method was applied to plasma samples from children. Conclusion: The developed LC–MS/MS method is suitable for pediatric studies with small volume plasma samples collected via capillary tubes. One limitation was the performance of PFP columns varied among different brands.
- Subjects :
- Clinical Biochemistry
Analytical chemistry
Analytic Sample Preparation Methods
Atmospheric-pressure chemical ionization
Tandem mass spectrometry
Article
Analytical Chemistry
Antimalarials
Tandem Mass Spectrometry
Piperaquine
Lc ms ms
Humans
General Pharmacology, Toxicology and Pharmaceutics
Child
Pediatric
Chromatography
Liquid
Clinical Trials as Topic
Plasma samples
Chemistry
Small volume
Prevention
Small sample
Pharmacology and Pharmaceutical Sciences
General Medicine
Medical Laboratory Technology
Good Health and Well Being
Quinolines
Blood Chemical Analysis
Chromatography, Liquid
Subjects
Details
- ISSN :
- 17576199 and 17576180
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Bioanalysis
- Accession number :
- edsair.doi.dedup.....33fc9f3c3d1e2752a10d09a4bff010a3