Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:05:03Z No. of bitstreams: 1 S1415-47572009000100024.pdf: 81043 bytes, checksum: 04d9c31a7cba9d5aa733af9fb52b9ad1 (MD5) Made available in DSpace on 2013-08-22T19:05:03Z (GMT). No. of bitstreams: 1 S1415-47572009000100024.pdf: 81043 bytes, checksum: 04d9c31a7cba9d5aa733af9fb52b9ad1 (MD5) Previous issue date: 2009-01-01 Made available in DSpace on 2013-09-30T20:08:42Z (GMT). No. of bitstreams: 2 S1415-47572009000100024.pdf: 81043 bytes, checksum: 04d9c31a7cba9d5aa733af9fb52b9ad1 (MD5) S1415-47572009000100024.pdf.txt: 21127 bytes, checksum: 98afd83ff92a8a77363ba5b1fb75f38b (MD5) Previous issue date: 2009-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:54:50Z No. of bitstreams: 2 S1415-47572009000100024.pdf: 81043 bytes, checksum: 04d9c31a7cba9d5aa733af9fb52b9ad1 (MD5) S1415-47572009000100024.pdf.txt: 21127 bytes, checksum: 98afd83ff92a8a77363ba5b1fb75f38b (MD5) Made available in DSpace on 2014-05-20T13:54:50Z (GMT). No. of bitstreams: 2 S1415-47572009000100024.pdf: 81043 bytes, checksum: 04d9c31a7cba9d5aa733af9fb52b9ad1 (MD5) S1415-47572009000100024.pdf.txt: 21127 bytes, checksum: 98afd83ff92a8a77363ba5b1fb75f38b (MD5) Previous issue date: 2009-01-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação para o Desenvolvimento da UNESP (FUNDUNESP) The present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence between silver and ethidium bromide staining. Comets were visually examined and, the frequency of cells with and without damage was obtained, as well as the distribution of classes and scores. By using the Kruskal-Wallis test, our results revealed that pH 12.1 is more effective, although both pHs can be used. Our findings also suggest that silver staining can substitute ethidium bromide, an expensive and highly toxic stain that requires specific equipment for examination. Universidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Departamento de Biologia Universidade Federal do Pará (UFPA) Instituto de Biociências Departamento de Genética Universidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Departamento de Biologia