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Methyl palmitate modulates the nicotine‐induced increase in basilar arterial blood flow
- Source :
- Microcirculation. 28
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Methyl palmitate (MP) is a fatty acid methyl ester. Our recent study indicated that adrenergic nerve-dependent functional sympathetic-sensory nerve interactions were abolished by MP in mesenteric arteries. However, the effect of MP on perivascular nerves and cerebral blood flow remains unclear. In this study, the increase in basilar arterial blood flow (BABF) after the topical application of nicotinic acetylcholine receptor agonists was measured using laser Doppler flowmetry in anesthetized rats. The choline (a selective α7-nicotinic acetylcholine receptor agonist)-induced increase in BABF was abolished by tetrodotoxin (a neurotoxin), NG -nitro-L-arginine (a nonselective NO synthase inhibitor), α-bungarotoxin (a selective α7-nicotinic acetylcholine receptor inhibitor), and chronic sympathetic denervation. In addition, the nicotine (a nicotinic acetylcholine receptor agonist)-induced increase in BABF was inhibited by MP in a concentration-dependent manner. The acetylcholine-induced increase in BABF was not affected by MP. The myography results revealed that nicotine-induced vasorelaxation was significantly inhibited by MP, but was reversed by chelerythrine (a protein kinase C inhibitor). MP-induced vasodilation was significantly greater in BA rings without endothelium compared to those with endothelium. Meanwhile, MP did not affect baseline BABF. Our results indicate that MP acts as a neuromodulator in the cerebral circulation where it activates the PKC pathway and causes a diminished nicotine-induced increase in blood flow in the brainstem, and that the vasorelaxation effect of MP may play a minor role.
- Subjects :
- Male
Agonist
Nicotine
Physiology
medicine.drug_class
Palmitates
Vasodilation
Receptors, Nicotinic
030204 cardiovascular system & hematology
Pharmacology
03 medical and health sciences
Cerebral circulation
chemistry.chemical_compound
0302 clinical medicine
Physiology (medical)
Laser-Doppler Flowmetry
medicine
Animals
Neurotoxin
Nicotinic Agonists
Molecular Biology
Mesenteric arteries
Acetylcholine receptor
Neurotransmitter Agents
Chemistry
Rats
Nicotinic acetylcholine receptor
medicine.anatomical_structure
Chelerythrine
Basilar Artery
Cardiology and Cardiovascular Medicine
030217 neurology & neurosurgery
Brain Stem
Subjects
Details
- ISSN :
- 15498719 and 10739688
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Microcirculation
- Accession number :
- edsair.doi.dedup.....343631d881a5eb602384e05605616199