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Biochemical, Histopathological and Molecular Responses in Gills of Leuciscus cephalus Exposed to Metals
- Source :
- Archives of Environmental Contamination and Toxicology. 73:607-618
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Gills are major targets for acute metal toxicity in fish, due to their permanent contact with aquatic pollutants. To assess the effects of metals on gills of the Leuciscus cephalus (chub), fish individuals were collected from two sites in the Tur River, Romania, in upstream (site 1) and downstream (site 2) of a metal pollution source. Quantitative and hyperspectral analyses showed that Zn, Sr, and Fe concentrations were significantly higher in gills from site 2 compared with site 1. Malondialdehyde and advanced oxidation protein products levels increased 17 and 28%, respectively, whereas reduced glutathione level diminished significantly in the gills of fish collected from site 2 compared to site 1. The activities of superoxide dismutase, catalase, and glutathione-S-transferase increased significantly at 41, 21, and 28%, respectively. Proliferating cell nuclear antigen (PCNA) protein levels, as well as the amount of DNA damage, were significantly increased for site 2 compared with site 1. The induced oxidative stress generated hyperplasia, hypertrophy, and inflammation in the epithelial cells and apoptosis. Hence, this could suggest that gill cells have tried to counteract the oxidative stress-induced DNA fragmentation by PCNA up-regulation, but the PCNA expression decreased on longer time due to the low level of GSH, resulting in apoptosis.
- Subjects :
- Gills
0301 basic medicine
Gill
Health, Toxicology and Mutagenesis
Cyprinidae
010501 environmental sciences
Toxicology
medicine.disease_cause
01 natural sciences
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
Leuciscus
Természettudományok
Malondialdehyde
medicine
Animals
Kémiai tudományok
Glutathione Transferase
0105 earth and related environmental sciences
biology
Superoxide Dismutase
General Medicine
Glutathione
biology.organism_classification
Pollution
Molecular biology
Proliferating cell nuclear antigen
030104 developmental biology
chemistry
Metals
Catalase
biology.protein
Water Pollutants, Chemical
Oxidative stress
Subjects
Details
- ISSN :
- 14320703 and 00904341
- Volume :
- 73
- Database :
- OpenAIRE
- Journal :
- Archives of Environmental Contamination and Toxicology
- Accession number :
- edsair.doi.dedup.....344908992e9457ec82b97c8766977b98