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The Autographa californica Multiple Nucleopolyhedrovirus ORF78 Is Essential for Budded Virus Production and General Occlusion Body Formation
- Source :
- Journal of Virology. 87:8441-8450
- Publication Year :
- 2013
- Publisher :
- American Society for Microbiology, 2013.
-
Abstract
- ORF78 ( ac78 ) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a baculovirus core gene of unknown function. To determine the role of ac78 in the baculovirus life cycle, an AcMNPV mutant with ac78 deleted, Ac78KO, was constructed. Quantitative PCR analysis revealed that ac78 is a late gene in the viral life cycle. After transfection into Spodoptera frugiperda cells, Ac78KO produced a single-cell infection phenotype, indicating that no infectious budded viruses (BVs) were produced. The defect in BV production was also confirmed by both viral titration and Western blotting. However, viral DNA replication was unaffected, and occlusion bodies were formed. An analysis of BVs and occlusion-derived viruses (ODVs) revealed that AC78 is associated with both forms of the virions and is an envelope structural protein. Electron microscopy revealed that AC78 also plays an important role in the embedding of ODV into the occlusion body. The results of this study demonstrate that AC78 is a late virion-associated protein and is essential for the viral life cycle.
- Subjects :
- viruses
Intranuclear Inclusion Bodies
Molecular Sequence Data
Immunology
Mutant
Spodoptera
Biology
Virus Replication
Microbiology
Virus
Viral Proteins
Microscopy, Electron, Transmission
Viral life cycle
Virology
Sf9 Cells
Animals
Virus Release
Cell Nucleus
Genes, Essential
Gene Expression Profiling
fungi
Sequence Analysis, DNA
Transfection
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Molecular biology
Nucleopolyhedroviruses
Genome Replication and Regulation of Viral Gene Expression
Gene expression profiling
Autographa californica
Viral replication
Insect Science
DNA, Viral
Gene Deletion
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 87
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....3470e553da6b5be4d968547e0a6609cf