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Effect of Alkylresorcinols on Autophagy, Migration, and Invasion of HepG2 Cells
- Source :
- Journal of Food Science. 84:3063-3068
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Alkylresorcinols are phenolic lipids that mainly exist in the cortex of grains, and they exhibit anticancer activity against various cancer cells in vitro. However, the underlying action mechanisms are still unclear. In our study, the influence of alkylresorcinols C19:0 and C21:0 (ARs) upon migration, invasion, and autophagy in human hepatoblastoma HepG2 cells was evaluated. Results showed that ARs at 80 and 160 µg/mL significantly suppressed cells proliferation, migration, and invasion, downregulated the expression of proteins RhoA and MMP-7 associated with migration and invasion. ARs at 160 µg/mL, the rate of LC3 puncta was appreciably increased. After autophagy was blocked by 3-MA or CQ, the expression of LC3II was significantly increased in 3-MA+ARs group and p62 was significantly decreased in CQ+ARs group. The results indicate that ARs may promote autophagic flow. ARs (80, 160 µg/mL) significantly inhibited the expression of proteins p-mTOR, p-PI3K, and p-Akt related to the PI3K/Akt pathway. The results of the present study suggest that ARs can activate autophagy and suppresses the biological behaviors of HepG2 cells by inhibiting the activation of MMP-7, Rho/Rho-associated protein kinase, and activation of the phosphatidylinositol 3-kinase/Akt signaling pathway. PRACTICAL APPLICATION: The anticancer mechanism of ARs in wheat bran was studied, which provided a basis for the development of anticancer functional auxiliary food with wheat bran as raw material. It is of great practical significance to promote the effective utilization of grain processing by-products and improve the economic benefits of the grain industry.
- Subjects :
- RHOA
030309 nutrition & dietetics
Apoptosis
03 medical and health sciences
0404 agricultural biotechnology
Cell Movement
Autophagy
Humans
Protein kinase A
PI3K/AKT/mTOR pathway
Cell Proliferation
0303 health sciences
Molecular Structure
biology
Kinase
Chemistry
Akt/PKB signaling pathway
TOR Serine-Threonine Kinases
Hep G2 Cells
Resorcinols
04 agricultural and veterinary sciences
040401 food science
Cell biology
Cell culture
Cancer cell
biology.protein
Phosphatidylinositol 3-Kinase
Proto-Oncogene Proteins c-akt
Signal Transduction
Food Science
Subjects
Details
- ISSN :
- 17503841 and 00221147
- Volume :
- 84
- Database :
- OpenAIRE
- Journal :
- Journal of Food Science
- Accession number :
- edsair.doi.dedup.....34d44f819022eece3cb9ce9063969df1