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Identification of Thyroid-Associated Serum microRNA Profiles and Their Potential Use in Thyroid Cancer Follow-Up
- Source :
- Journal of the Endocrine Society
- Publication Year :
- 2017
- Publisher :
- ENDOCRINE SOC, 2017.
-
Abstract
- Context: Trends toward more conservative management of papillary thyroid cancer (PTC) diminish the primacy of serum thyroglobulin (Tg) assays as a posttreatment surveillance tool. Objective: To identify thyroid tumor-associated microRNAs (miRNAs) in the serum with potential for development as unique biomarkers of PTC recurrence. Methods: We measured expression of 754 miRNAs in serum samples collected from 11 patients with PTC before and 30 days after thyroidectomy. Major candidates were then re-evaluated by absolute quantitative polymerase chain reaction analysis in an independent cohort of patients with PTC (n = 44) or benign nodules and 20 healthy controls (HCs). The 2 miRNAs most significantly associated with thyroid tumors were then assessed in matched serum samples (before and 30 days and 1 to 2 years after surgery) from the 20 PTC patients with complete follow-up datasets and results correlated with American Thyroid Association (ATA) responses to therapy. Results: Eight miRNAs (miR-221-3p, miR-222-3p, miR-146a-5p, miR-24-3p, miR-146b-5p, miR-191-5p, miR-103a-3p, and miR-28-3p) displayed levels in prethyroidectomy serum samples from patients with PTC that significantly exceeded those measured after thyroidectomy and those found in samples from HCs. The 2 most promising candidates—miR-146a-5p and miR-221-3p —were further analyzed in the 20 PTC patients mentioned earlier. Serum levels of both miRNAs after 1 to 2 years of follow-up were consistent with ATA responses to therapy in all patients, including 2 with structural evidence of disease whose Tg assays remained negative (<br />Precis: Analysis of miRNA levels in pre- and postoperative serum samples from PTC patients reveals possible associations between postoperative changes in miR-146a-5p and miR-221-3p and clinical outcome.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Pathology
endocrine system diseases
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
Settore MED/18 - CHIRURGIA GENERALE
Context (language use)
Gastroenterology
Papillary thyroid cancer
03 medical and health sciences
Internal medicine
medicine
follow-up
Thyroid cancer
circulating
Thyroid
microRNA
business.industry
Thyroidectomy
medicine.disease
Editor's Choice
030104 developmental biology
Real-time polymerase chain reaction
medicine.anatomical_structure
Cohort
papillary thyroid carcinoma
Biomarker (medicine)
biomarker
business
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of the Endocrine Society
- Accession number :
- edsair.doi.dedup.....34d71d9d5289c11f3e5206b9e6c4005c