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Interaction of ovarian steroidogenesis and clock gene expression modulated by bone morphogenetic protein-7 in human granulosa cells
- Source :
- Endocrine Journal. 66:157-164
- Publication Year :
- 2019
- Publisher :
- Japan Endocrine Society, 2019.
-
Abstract
- A functional link between clock gene expression and ovarian steroidogenesis was studied using human granulosa KGN cells. Similarities between changes in the mRNA and protein expression levels of Bmal1 and Clock and those of Per2 and Cry1 were found in KGN cells after treatment with forskolin. Among the interrelationships between the expression levels of clock and steroidogenic factors, Clock mRNA had a strongly positive correlation with P450arom and a negative correlation with 3βHSD. Knockdown of Clock gene by siRNA resulted in a significant reduction of estradiol production by inhibiting P450arom expression, while it induced a significant increase of progesterone production by upregulating 3βHSD in KGN cells treated with forskolin. Moreover, BMP-7 had an enhancing effect on the expression of Clock mRNA and protein in KGN cells. Thus, the expression levels of Clock, being upregulated by forskolin and BMP-7, were functionally linked to estradiol production and progesterone suppression by human granulosa cells.
- Subjects :
- endocrine system
3-Hydroxysteroid Dehydrogenases
Bone Morphogenetic Protein 7
Endocrinology, Diabetes and Metabolism
CLOCK Proteins
Gene Expression
030209 endocrinology & metabolism
Positive correlation
03 medical and health sciences
chemistry.chemical_compound
Aromatase
0302 clinical medicine
Endocrinology
Downregulation and upregulation
Cell Line, Tumor
Humans
Progesterone
Gene knockdown
Messenger RNA
Granulosa Cells
Forskolin
Estradiol
Colforsin
Cell biology
PER2
Bone morphogenetic protein 7
CLOCK
chemistry
030220 oncology & carcinogenesis
Female
Subjects
Details
- ISSN :
- 13484540 and 09188959
- Volume :
- 66
- Database :
- OpenAIRE
- Journal :
- Endocrine Journal
- Accession number :
- edsair.doi.dedup.....34f6fe416bfe6c255935e96fbba46704