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R-Ras regulates vascular permeability, but not overall healing in skin wounds
- Source :
- Experimental Dermatology. 28:202-206
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Wounds close by keratinocytes migrating from the edge of the wound and re-epithelializing the epidermis. It has been proposed that the major stimuli for wound closure are blood-derived growth factors, chemokines and cytokines. The small GTPase R-Ras, a known integrin activator, also regulates vascular permeability during angiogenesis, and blood vessels lacking R-Ras leak plasma proteins constantly. We explored whether the access to blood-derived proteins influences skin wound healing in R-Ras knockout (KO) mice. In skin wounds, R-Ras expression was mostly restricted to the vasculature in the granulation tissue. Angiogenic blood vessels in the R-Ras KO mice were significantly more permeable than in wild-type (WT) controls. Although the distances between epidermal tongues, and the panniculus carnosus muscles, were significantly longer in R-Ras KO than WT controls before the granulation tissue formation took place, there were no differences in the wound closure or re-epithelialization rates or granulation tissue formation. These findings were also corroborated in a special splint excision wound model. Our study shows that although R-Ras does not influence the skin wound healing itself, the blood vessels lacking R-Ras are leaky and thus could facilitate the access of blood-derived proteins to the wound.
- Subjects :
- Keratinocytes
Male
0301 basic medicine
Integrins
Chemokine
Pathology
Angiogenesis
Vascular permeability
Biochemistry
angiogenesis
Mice
030207 dermatology & venereal diseases
0302 clinical medicine
Re-Epithelialization
Cell Movement
Skin
Mice, Knockout
Neovascularization, Pathologic
integumentary system
biology
R-Ras
Sisätaudit - Internal medicine
Granulation tissue
Blood proteins
Panniculus carnosus
medicine.anatomical_structure
Female
Guanosine Triphosphate
medicine.medical_specialty
integrin
Biolääketieteet - Biomedicine
wound
Dermatology
Skin Diseases
Capillary Permeability
03 medical and health sciences
medicine
Animals
vascular permeability
Molecular Biology
Wound Healing
Epidermis (botany)
business.industry
Activator (genetics)
Microcirculation
Mice, Inbred C57BL
030104 developmental biology
ras Proteins
biology.protein
Epidermis
business
Subjects
Details
- ISSN :
- 09066705
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Experimental Dermatology
- Accession number :
- edsair.doi.dedup.....351a8b97d72d6d44b4089058104fb375