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Identification of a peroxisome proliferator responsive element (PPRE)-like cis-element in mouse plasminogen activator inhibitor-1 gene promoter
- Source :
- Biochemical and Biophysical Research Communications. 347:821-826
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- PAI-1 is expressed and secreted by adipose tissue which may mediate the pathogenesis of obesity-associated cardiovascular complications. Evidence is presented in this report that PAI-1 is not expressed by preadipocyte, but significantly induced during 3T3-L1 adipocyte differentiation and the PAI-1 expression correlates with the induction of peroxisome proliferator-activated receptor gamma (PPARgamma). A peroxisome proliferator responsive element (PPRE)-like cis-element (-206TCCCCCATGCCCT-194) is identified in the mouse PAI-1 gene promoter by electrophoretic mobility shift assay (EMSA) combined with transient transfection experiments; the PPRE-like cis-element forms a specific DNA-protein complex only with adipocyte nuclear extracts, not with preadipocyte nuclear extracts; the DNA-protein complex can be totally competed away by non-labeled consensus PPRE, and can be supershifted with PPARgamma antibody. Mutation of this PPRE-like cis-element can abolish the transactivation of mouse PAI-1 promoter mediated by PPARgamma. Specific PPARgamma ligand Pioglitazone can significantly induce the PAI-1 expression, and stimulate the secretion of PAI-1 into medium.
- Subjects :
- Transcription, Genetic
Biophysics
Adipose tissue
Biology
Ligands
Response Elements
Biochemistry
Mice
chemistry.chemical_compound
Transactivation
3T3-L1 Cells
Adipocyte
Plasminogen Activator Inhibitor 1
Adipocytes
Animals
Electrophoretic mobility shift assay
Promoter Regions, Genetic
Receptor
Molecular Biology
Cell Differentiation
Promoter
3T3-L1
Cell Biology
Molecular biology
PPAR gamma
chemistry
Plasminogen activator inhibitor-1
Peroxisome Proliferators
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 347
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....351c0f40461d1f953da44ae1cd94e129