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Mycoplasma species isolated from bovine milk collected from US dairy herds between 2016 and 2019
- Source :
- Journal of Dairy Science. 104:4813-4821
- Publication Year :
- 2021
- Publisher :
- American Dairy Science Association, 2021.
-
Abstract
- Determining the species of mycoplasma isolated from culture-positive milk samples is important for understanding the clinical significance of their detection. Between August 2016 and December 2019, 214,518 milk samples from 2,757 dairy herds were submitted to Quality Milk Production Services (QMPS) at Cornell University for mycoplasma culture. From these samples, 3,728 collected from 204 herds were culture positive. Based on the request of herd managers, owners, or veterinarians, 889 isolates from 98 herds were subjected to molecular identification by PCR and amplicon sequencing. The largest proportion of the identified isolates were from New York State (78.1%), while the others came from the eastern United States (17.8%), Texas (2.0%), and New Mexico (2.1%). As expected, Mycoplasma spp. were the most common (855 isolates, 96.2%) and Acholeplasma spp. accounted for the remainder (34 isolates, 3.8%). Mycoplasma bovis was the most prevalent Mycoplasma species (75.1%), followed by M. bovigenitalium (6.5%), M. canadense (5.9%), M. alkalescens (5%), M. arginini (1.7%), M. californicum (0.1%), and M. primatum (0.1%). A portion of the isolates were confirmed as Mycoplasma spp. other than M. bovis but were not identified at the species level (16 isolates, 1.8%) because further information was not requested by the manager, owner, or veterinarian. Mycoplasma bovis was the only species identified in 59 of the 98 herds. However, more than 1 Mycoplasma sp. was identified in 29 herds, suggesting that herd infection with 2 or more mycoplasmas is not uncommon. Moreover, a Mycoplasma sp. other than M. bovis was the only species identified in 8 herds. From the subset of 889 mycoplasma culture-positive isolates from 98 herds, we determined that over a third of the herds had either more than 1 Mycoplasma sp. or a Mycoplasma sp. other than M. bovis detected in their milk samples. In conclusion, we observed that M. bovis is the most common pathogenic Mycoplasma species found in mastitic milk, but other Mycoplasma species are not uncommon. Our results suggest that it is critical to test milk samples for mycoplasmas using diagnostic tests able to identify both the genus and the species.
- Subjects :
- Mycoplasma bovis
Veterinary medicine
New York
Mycoplasma species
Biology
medicine.disease_cause
03 medical and health sciences
Genetics
medicine
Animals
Mycoplasma Infections
Mastitis, Bovine
030304 developmental biology
Molecular identification
0303 health sciences
Dairy herds
0402 animal and dairy science
04 agricultural and veterinary sciences
Mycoplasma
medicine.disease
biology.organism_classification
Texas
040201 dairy & animal science
Mastitis
Acholeplasma
Milk
Herd
Cattle
Female
Animal Science and Zoology
Food Science
Subjects
Details
- ISSN :
- 00220302
- Volume :
- 104
- Database :
- OpenAIRE
- Journal :
- Journal of Dairy Science
- Accession number :
- edsair.doi.dedup.....3520a75b41faa7c9ebba221e15ea4c58
- Full Text :
- https://doi.org/10.3168/jds.2020-19171