Back to Search
Start Over
Proteomic studies on the white matter of human brain
- Source :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 833(1)
- Publication Year :
- 2005
-
Abstract
- Limited information on the protein expression profiles of the different components of mammalian brain is available to date. In the present study, proteomic analysis was performed on 32 white matter samples obtained from 8 different regions of brains of four post mortem cases. Proteins were separated by 2D gel electrophoresis and identified by mass spectrometry. Most of the protein spots (98%) are reproducibly present in all the samples analyzed. A total of 64 different proteins were identified and divided into seven functional groups. These include metabolic proteins (33%), structural proteins (9%), proteins involved in signal transduction (9%), blood proteins (8%), stress related proteins (23%), and proteins involved in the ubiquitin mediated proteolysis (6%). This protein database obtained from the white matter of human brain contributes to deepen our knowledge on the molecular mechanisms that control several pathologies affecting this key component of the brain.
- Subjects :
- Proteomics
Proteolysis
Clinical Biochemistry
Biochemistry
Mass Spectrometry
Analytical Chemistry
White matter
Ubiquitin
medicine
Humans
Electrophoresis, Gel, Two-Dimensional
Two-dimensional gel electrophoresis
Chromatography
medicine.diagnostic_test
biology
Chemistry
Brain
Cell Biology
General Medicine
Human brain
Blood proteins
Molecular biology
medicine.anatomical_structure
biology.protein
Signal transduction
Subjects
Details
- ISSN :
- 15700232
- Volume :
- 833
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
- Accession number :
- edsair.doi.dedup.....352ddd595e7c4fd33beb3b110ede54a6