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Extended-spectrum beta-lactamase (ESBL)-producing and non-ESBL-producing Escherichia coli isolates causing bacteremia in the Netherlands (2014 - 2016) differ in clonal distribution, antimicrobial resistance gene and virulence gene content
- Source :
- PLoS ONE, Vol 15, Iss 1, p e0227604 (2020), PLoS ONE, PLoS ONE, 15(1). Public Library of Science
- Publication Year :
- 2020
- Publisher :
- Public Library of Science (PLoS), 2020.
-
Abstract
- BackgroundKnowledge on the molecular epidemiology of Escherichia coli causing E. coli bacteremia (ECB) in the Netherlands is mostly based on extended-spectrum beta-lactamase-producing E. coli (ESBL-Ec). We determined differences in clonality and resistance and virulence gene (VG) content between non-ESBL-producing E. coli (non-ESBL-Ec) and ESBL-Ec blood isolates with different epidemiological characteristics.Materials/methodsA random selection of non-ESBL-Ec isolates as well as all available ESBL-Ec blood isolates was obtained from two Dutch hospitals between 2014 and 2016. Whole genome sequencing was performed to infer sequence types (STs), serotypes, acquired antibiotic resistance genes and VG scores, based on presence of 49 predefined putative pathogenic VG.ResultsST73 was most prevalent among the 212 non-ESBL-Ec (N=26, 12.3%) and ST131 among the 69 ESBL-Ec (N=30, 43.5%). Prevalence of ST131 among non-ESBL-Ec was 10.4% (N=22, P value < 0.001 compared to ESBL-Ec). O25:H4 was the most common serotype in both non-ESBL-Ec and ESBL-Ec. Median acquired resistance gene counts were 1 (IQR 1 – 6) and 7 (IQR 4 – 9) for non-ESBL-Ec and ESBL-Ec, respectively (P value < 0.001). Among non-ESBL-Ec, acquired resistance gene count was highest among blood isolates from a primary gastro-intestinal focus (median 4, IQR 1 – 8). Median VG scores were 13 (IQR 9 – 20) and 12 (IQR 8 – 14) for non-ESBL-Ec and ESBL-Ec, respectively (P value = 0.002). VG scores among non-ESBL-Ec from a primary urinary focus (median 15, IQR 11 – 21) were higher compared to non-ESBL-Ec from a primary gastro-intestinal (median 10, IQR 6 – 13) or hepatic-biliary focus (median 11, IQR 5 – 18) (P values = 0.007 and 0.036, respectively). VG content varied between different E. coli STs.ConclusionsNon-ESBL-Ec and ESBL-Ec blood isolates from two Dutch hospitals differed in clonal distribution, resistance gene and VG content. Also, resistance gene and VG content differed between non-ESBL-Ec from different primary foci of ECB.
- Subjects :
- Bacterial Diseases
0301 basic medicine
Serotype
Physiology
Epidemiology
medicine.medical_treatment
Bacteremia
Antimicrobial resistance
Pathology and Laboratory Medicine
medicine.disease_cause
Blood counts
Biochemistry
Geographical locations
Medicine and Health Sciences
Prevalence
polycyclic compounds
Genetic epidemiology
Escherichia coli Infections
Netherlands
Escherichia Coli
Vaccines
Molecular Epidemiology
Multidisciplinary
Agricultural and Biological Sciences(all)
Virulence
Body Fluids
Bacterial Pathogens
Anti-Bacterial Agents
Europe
Blood
Infectious Diseases
Experimental Organism Systems
Medical Microbiology
Genetic Epidemiology
Prokaryotic Models
Medicine
Anatomy
Pathogens
Research Article
Escherichia
Infectious Disease Control
Science
030106 microbiology
Microbial Sensitivity Tests
Biology
Research and Analysis Methods
Microbiology
beta-Lactamases
03 medical and health sciences
Model Organisms
Antibiotic resistance
Enterobacteriaceae
Microbial Control
Drug Resistance, Bacterial
Escherichia coli
medicine
Humans
European Union
General
Microbial Pathogens
Gene
Pharmacology
Bacteria
Molecular epidemiology
Biochemistry, Genetics and Molecular Biology(all)
Gut Bacteria
Organisms
Biology and Life Sciences
biochemical phenomena, metabolism, and nutrition
medicine.disease
bacterial infections and mycoses
Blood Counts
030104 developmental biology
Animal Studies
Beta-lactamase
bacteria
Antimicrobial Resistance
People and places
Genetics and Molecular Biology(all)
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 15
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....3536d2508793e77377c300faef4cbf20